FLUORESCENCE MICROSCOPY OF PROTEINS INVOLVED IN GOLGI-TO-VACUOLE VESICLE TRAFFIC

参与高尔基体到液泡囊泡运输的蛋白质的荧光显微镜

• 批准号: 7723724
• 负责人: Alexey Jarrell Merz
• 金额: $ 1.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723724
• 关键词: Coated vesicle; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Microscopy; Funding; Goals; Golgi Apparatus; Grant; In Vitro; Institution; Pathway interactions; Proteins; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; Vacuole; Vesicle; in vivo; insight; interest; trafficking; vesicle/vacuole

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the role of known vacuole fusion factors within the AP-3 vesicle trafficking pathway. While we have identified in vitro interactions between the AP-3 vesicle coat complex and a subset of fusion factors, whether these interactions occur in vivo remains unknown. Our goal is to use fluorescence microscopy to determine the colocalization of AP-3 with these fusion factors in vivo and at which cellular compartment this may take place. This information will provide insight into whether AP-3 interacts with the fusion factors in vivo and the possible function of these interactions.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们对已知的液泡融合因子在 AP-3 囊泡运输途径中的作用感兴趣。虽然我们已经在体外鉴定了 AP-3 囊泡外壳复合物和融合因子子集之间的相互作用，但这些相互作用是否在体内发生仍然未知。我们的目标是使用荧光显微镜来确定 AP-3 与这些融合因子在体内的共定位以及这可能发生在哪个细胞区室。该信息将深入了解 AP-3 是否与体内融合因子相互作用以及这些相互作用的可能功能。