CHARACTERIZATION OF THE NUCLEAR PORECOMPLEX OF THE AFRICAN TRYPANOSOME

非洲锥虫核孔复合物的特征

• 批准号: 8169120
• 负责人: Brian T Chait
• 金额: $ 2.33万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169120
• 关键词: African; Architecture; Bioinformatics; Cell Nucleus; Coated vesicle; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; Eukaryota; Evolution; Funding; Genomics; Grant; Institution; Left; Manuscripts; Mediating; Molecular; Nuclear; Nuclear Pore Complex; Proteomics; Publishing; Research; Research Personnel; Resources; Route; Source; Trypanosoma; United States National Institutes of Health; Work; macromolecular assembly; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The nuclear pore complex (NPC) is a massive macromolecular assembly, unique to Eukaryotes, that mediates bidirectional exchange of material between the nucleus and cytoplasm. Until recently, the evolutionary origin of the NPC was unknown, leaving a major gap in our understanding of the origin of the Eukaryota. Prior bioinformatic studies led to the conclusion that the Last Common Eukaryotic Ancestor (LCEA) possessed a rather primitive ancestral NPC, passing few components to its highly divergent modern descendants. Using a proteomic/genomic approach, we show that the LCEA actually possessed an NPC with a surprisingly modern architecture. Moreover, we have established that all NPCs have a fundamentally similar scaffold architecture that resembles the architecture of coated vesicles. Our findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes, and that both were established very early in eukaryotic evolution. A manuscript describing this work has been published (23. J.A. DeGrasse, K.N. DuBois, D. Devos, T.N. Siegel, A. Sali, M.C. Field, M.P. Rout, B.T. Chait "Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor" Molecular & Cellular Proteomics, 8 (2009) 2119-30).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 核孔复合体（NPC）是真核生物特有的巨大大分子组装体，介导细胞核和细胞质之间的双向物质交换。直到最近，NPC 的进化起源仍不清楚，这给我们对真核生物起源的理解留下了重大空白。先前的生物信息学研究得出的结论是，最后的共同真核祖先（LCEA）拥有相当原始的祖先 NPC，将很少的成分传递给其高度分化的现代后代。使用蛋白质组/基因组方法，我们证明 LCEA 实际上拥有一个具有令人惊讶的现代建筑的 NPC。此外，我们已经确定所有 NPC 都具有基本相似的支架结构，类似于包被囊泡的结构。我们的研究结果强烈支持这样的假设：NPC 与囊泡涂层复合物具有共同的祖先，并且两者都是在真核进化的早期建立的。描述这项工作的手稿已发表（23. J.A. DeGrasse、K.N. DuBois、D. Devos、T.N. Siegel、A. Sali、M.C. Field、M.P. Rout、B.T. Chait“共享核孔复合体结构的证据，该结构是从最后的共同真核祖先”《分子与细胞蛋白质组学》，8 (2009) 2119-30）。