PROTEIN INTERACTIONS IN YEAST VACUOLE FUSION

酵母液泡融合中的蛋白质相互作用

• 批准号: 7420670
• 负责人: Alexey Jarrell Merz
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420670
• 关键词: proteins; yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the roles of several proteins involved in yeast vacuole membrane fusion, including a Rab GTPase (Ypt7p), its effector complex (HOPS), and vacuolar SNARE proteins (Vam3p, Vam7p, Vti1p, Nyv1p). We are generating 2-hybrid baits that include putative subdomains of these proteins identified through bioinformatic analyses. Our goal is to generate a protein interaction map of known docking and fusion proteins, and to identify new interactors.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。我们对参与酵母液泡膜融合的几种蛋白质的作用感兴趣，包括 Rab GTPase (Ypt7p)、其效应复合物 (HOPS) 和液泡 SNARE 蛋白 (Vam3p、Vam7p、Vti1p、Nyv1p)。我们正在生成 2 种混合诱饵，其中包括通过生物信息学分析识别的这些蛋白质的推定子域。我们的目标是生成已知对接和融合蛋白的蛋白质相互作用图，并识别新的相互作用物。