MOLECULAR ARCHITECTURE OF NUCLEAR PORE COMPLEX (NPC) FROM YEAST S CEREVISIAE

酿酒酵母核孔复合物 (NPC) 的分子结构

• 批准号: 7954053
• 负责人: MICHAEL P ROUT
• 金额: $ 4.74万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954053
• 关键词: Architecture; Coated vesicle; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Disease; Employee Strikes; Face; Funding; Grant; Institution; Membrane; Methods; Molecular; Nature; Nuclear Envelope; Nuclear Pore Complex; Paper; Proteins; Proteomics; Publishing; Research; Research Personnel; Resources; Route; Source; Structure; Surface; United States National Institutes of Health; Yeasts; macromolecular assembly; macromolecule; numb protein; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nuclear pore complexes (NPCs) are proteinaceous assemblies of approximately 50 MDa that selectively transport cargoes across the nuclear envelope. To determine the molecular architecture of the yeast NPC, we collected a diverse set of biophysical and proteomic data, and developed a method for using these data to localize the NPC's 456 constituent proteins. Our structure reveals that half of the NPC is made up of a core scaffold, which is structurally analogous to vesicle-coating complexes. This scaffold forms an interlaced network that coats the entire curved surface of the nuclear envelope membrane within which the NPC is embedded. The selective barrier for transport is formed by large numbers of proteins with disordered regions that line the inner face of the scaffold. The NPC consists of only a few structural modules that resemble each other in terms of the configuration of their homologous constituents, the most striking of these being a 16-fold repetition of 'columns'. These findings provide clues to the evolutionary origins of the NPC. These results were published in two papers: F.Alber, S. Dokudovskaya, L.M. Veenhoff, W. Zhang, J. Kipper, D. Devos, A. Suprapto, O. Karni-Schmidt, R. Williams, B.T. Chait, M.P. Rout, Andrej Sali Determining the architectures of macromolecular assemblies Nature 450 (2007) 683-694 F.Alber, S. Dokudovskaya, L.M. Veenhoff, W. Zhang, J. Kipper, D. Devos, A. Suprapto, O. Karni-Schmidt, R. Williams, B.T. Chait, M.P. Rout, Andrej Sali The molecular architecture of the nuclear pore complex Nature 450 (2007) 695-701

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 核孔复合物 (NPC) 是大约 50 MDa 的蛋白质组件，可选择性地跨核膜运输货物。为了确定酵母 NPC 的分子结构，我们收集了一组不同的生物物理和蛋白质组数据，并开发了一种使用这些数据定位 NPC 的 456 种组成蛋白的方法。我们的结构揭示了 NPC 的一半由核心支架组成，其结构类似于囊泡涂层复合物。该支架形成一个交错的网络，覆盖了 NPC 嵌入其中的核膜的整个弯曲表面。运输的选择性屏障是由大量蛋白质形成的，这些蛋白质具有排列在支架内表面的无序区域。 NPC 仅由几个结构模块组成，这些模块在同源成分的配置方面彼此相似，其中最引人注目的是 16 倍重复的“柱”。这些发现为 NPC 的进化起源提供了线索。这些结果发表在两篇论文中： F.Alber、S. Dokudovskaya、L.M. Veenhoff、W. 张、J. Kipper、D. Devos、A. Suprapto、O. Karni-Schmidt、R. Williams、B.T.柴特，M.P. Rout, Andrej Sali 确定大分子组装体的结构 Nature 450 (2007) 683-694 F.Alber、S. Dokudovskaya、L.M. Veenhoff、W. 张、J. Kipper、D. Devos、A. Suprapto、O. Karni-Schmidt、R. Williams、B.T.柴特，M.P. Rout, Andrej Sali 核孔复合体的分子结构 Nature 450 (2007) 695-701