IMPROVED METHOD TO LABEL PROTEINS WITH GFP

用 GFP 标记蛋白质的改进方法

• 批准号: 8171232
• 负责人: ERIC MULLER
• 金额: $ 8.43万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171232
• 关键词: Cellular biology; Cherry - dietary; Color; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Resonance Energy Transfer; Funding; Grant; Institution; Label; Measures; Methods; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Variant; improved; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to tag proteins with GFP and its color variants has revolutionized cell biology. In particular the CFP/YFP pair and the GFP/Cherry pair of fluorescent proteins make excellent FRET partners to measure the proximity of two proteins in vivo. We plan to develop a new method to tag proteins with the GFP variants.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 用 GFP 及其颜色变体标记蛋白质的能力彻底改变了细胞生物学。特别是荧光蛋白的 CFP/YFP 对和 GFP/Cherry 对构成了极好的 FRET 伙伴，可以测量体内两种蛋白的接近度。 我们计划开发一种用 GFP 变体标记蛋白质的新方法。