SOFTWARE DISTRIBUTED BY MULLER LAB, 2010

MULLER LAB 分发的软件，2010 年

• 批准号: 8171419
• 负责人: ERIC MULLER
• 金额: $ 1万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171419
• 关键词: Biosensor; Cells; Collection; Complex; Computer Retrieval of Information on Scientific Projects Database; Computer software; Fluorescence Resonance Energy Transfer; Funding; Grant; Institution; Life; Measures; Methodology; Monitor; Property; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; fluorophore; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fluorescence resonance energy transfer (FRET) between two fluorophores is a function of the distance between the fluorophores. This property is used experimentally to create sensors of biological activity and monitor changes in protein structure. FRET is also applied in living cells to measure the proximity of proteins within a complex that is tagged with a pair of fluorescent proteins. However this last application is hampered by the lack of a statistically robust methodology. FRETSCAL is an integrated collection of Matlab scripts for FRET analysis that provides the statistically robust methodology. Fluorescence resonance energy transfer (FRET) between two fluorophores is a function of the distance between the fluorophores. This property is used experimentally to create sensors of biological activity and monitor changes in protein structure. FRET is also applied in living cells to measure the proximity of proteins within a complex that is tagged with a pair of fluorescent proteins. However this last application is hampered by the lack of a statistically robust methodology. FRETSCAL is an integrated collection of Matlab scripts for FRET analysis that provides the statistically robust methodology.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 两个荧光团之间的荧光共振能量转移 (FRET) 是荧光团之间距离的函数。该特性在实验上用于创建生物活性传感器并监测蛋白质结构的变化。 FRET 还应用于活细胞，以测量标有一对荧光蛋白的复合物内蛋白质的接近度。然而，最后一个应用因缺乏统计上可靠的方法而受到阻碍。 FRETSCAL 是用于 FRET 分析的 Matlab 脚本的集成集合，提供了统计上稳健的方法。 两个荧光团之间的荧光共振能量转移 (FRET) 是荧光团之间距离的函数。这一特性在实验上用于创建生物活性传感器并监测蛋白质结构的变化。 FRET 还应用于活细胞中，以测量用一对荧光蛋白标记的复合物内蛋白质的接近度。然而，最后一个应用因缺乏统计上可靠的方法而受到阻碍。 FRETSCAL 是用于 FRET 分析的 Matlab 脚本的集成集合，提供了统计上稳健的方法。