DISSEMINATION OF STRAINS AND PLASMIDS BY THE MICROSCOPY GROUP

显微镜组传播菌株和质粒

• 批准号: 8171477
• 负责人: ERIC MULLER
• 金额: $ 2.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171477
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Microscopy; Open Reading Frames; Plasmids; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Venus; Yeasts; base; design; yeast protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The microscopy group previously constructed plasmids carrying CFP, YFP, Venus, and mCherry ORFs that express well in yeast. These plasmids allow fusion of fluorescent proteins to the C-terminus of any yeast protein by PCR-based integration. We altered a plasmid designed by Prein et al. so that YFP or CFP can be fused to the N-terminus of proteins by PCR-based integration. We also have over 1000 yeast strains containing tagged fluorescent proteins.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 显微镜小组先前构建了携带 CFP、YFP、Venus 和 mCherry ORF 在酵母中表达良好。这些质粒允许荧光蛋白融合 通过基于 PCR 的整合将其连接到任何酵母蛋白的 C 末端。我们改变了质粒 由 Prein 等人设计。这样YFP或CFP就可以通过以下方式融合到蛋白质的N末端 基于 PCR 的整合。 我们还有 1000 多种含有标记荧光的酵母菌株 蛋白质。