IMPROVED METHOD TO LABEL PROTEINS WITH GFP

用 GFP 标记蛋白质的改进方法

• 批准号: 8365922
• 负责人: ERIC MULLER
• 金额: $ 8.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365922
• 关键词: Biology; Cellular biology; Cherry - dietary; Color; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Grant; Label; Measures; Methods; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Variant; cost; improved; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to tag proteins with GFP and its color variants has revolutionized cell biology. In particular the CFP/YFP pair and the GFP/Cherry pair of fluorescent proteins make excellent FRET partners to measure the proximity of two proteins in vivo. We plan to develop a new method to tag proteins with the GFP variants.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 用GFP及其颜色变体标记蛋白质的能力彻底改变了细胞生物学。特别是CFP/YFP对和GFP/樱桃对荧光蛋白是出色的FRET伴侣，以测量体内两种蛋白质的接近度。 我们计划开发一种使用GFP变体标记蛋白质的新方法。