IMPROVED PROTOCOL TO STAIN THE CELL SURFACE, ACTIN AND DNA OF YEAST

改进的酵母细胞表面、肌动蛋白和 DNA 染色方案

• 批准号: 8365907
• 负责人: ERIC MULLER
• 金额: $ 10.41万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365907
• 关键词: Actins; Biology; Cell Cycle; Cell Shape; Cell surface; Cellular Morphology; Cytoskeleton; DNA; Funding; Fungal Genome; Grant; National Center for Research Resources; Phase; Principal Investigator; Protocols documentation; Publishing; Research; Research Infrastructure; Resources; Source; Staining method; Stains; Stream; United States National Institutes of Health; Yeasts; cost; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In order to capture a snapshot of the broad diversity among yeast strains we chose to examine gross cell morphology. In particular we wanted to examine the shape of the cells at different phases of the cell cycle, and the underlying actin distribution. Current published methods for staining the cell surface and actin cytoskeleton were neither robust nor stream-lined. Thus we developed new protocols.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 为了捕获酵母菌菌株之间广泛多样性的快照，我们选择了检查总细胞形态。特别是我们想检查细胞周期不同阶段的细胞的形状以及基础肌动蛋白分布。当前发表的用于染色细胞表面和肌动蛋白细胞骨架的方法既不稳健也不是衬里。因此，我们开发了新的协议。