Univ of Utah Cell Sorter for Flow Cytometry Core

犹他大学流式细胞仪核心细胞分选仪

• 批准号: 7793689
• 负责人: WAYNE FRANCIS GREEN
• 金额: $ 50万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-26 至 2011-01-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793689
• 关键词: Address; Area; Arts; Bacteria; Cancer Immunology Science; Cell Line; Cell Separation; Cells; Cellular biology; Cherry - dietary; Color; Data; Drug Delivery Systems; Financial compensation; Flow Cytometry; Fluorochrome; Funding; Genetic; Grant; Health; Health Sciences; Healthcare; Human; Image; Institutes; Lasers; Lymphoid Tissue; Malignant Neoplasms; Manufacturer Name; Medical; Medical center; National Center for Research Resources; Organelles; Peer Review; Pharmacy Schools; Population; Process; Public Health; Reporter; Research; Research Personnel; Resolution; Running; Sampling; Services; Sorting - Cell Movement; Technology; Testing; Time; United States National Institutes of Health; Universities; Utah; Yeasts; Zebrafish; drug development; instrument; instrumentation; meetings; public health relevance; red fluorescent protein; success; tumor

DESCRIPTION (provided by applicant): We propose replacing an existing FACSVantage SE cell sorter at the University of Utah's Health Sciences Center with a four laser (405, 488, 561 and 640 nm) FACSAria-II SORP cell sorter. The existing instrument was purchased in 1994 with NCRR funding and, despite institutionally funded upgrades, no longer meets the needs of investigators. The Vantage is limited to 6-color sorting but, lacking cross beam compensation, effective resolution of some fluorochromes is diminished by spill over making 6 colors technically impractical. The Vantage can only do two-way sorting, sample processing is relatively slow (4K-10K cells/sec depending on sample) and the increasingly common red fluorescent proteins (ex. m-Cherry) cannot be used. Currently, investigators are running 6-8 color analyses on newer flow cytometric analyzers on campus which cannot be replicated on the Vantage for cell sorting. Many investigators, dealing with limited sample material, want to sort three or four subpopulations which necessitate two sequential runs on the Vantage resulting in loss of some of the limited sample and increased time. Investigators using red fluorescent proteins as reporters are completely unable to sort cells on which they have already gathered imaging data using these probes. And finally, the manufacturer will no longer support the Vantage after 2010 making service and replacement parts problematic. The Vantage is located in a core lab and is the only shared use cell sorter on campus serving the Medical Center, Huntsman Cancer Institute, Eccles Genetics Institute, Howard Hughes Medical Institute, School of Pharmacy and undergraduate departments. A wide variety of sample material is analyzed and sorted ranging from bacteria, yeast and organelles in cellular lysates to zebra fish cells, lymphoid tissues, human tumor material and numerous cell lines. These services support NIH funded studies involving cancer, immunology, cell biology, drug development, drug delivery and public health. With the current instrumentation we are no longer able to provide state of the art cell sorting technology that is sought by many investigators which in turn impedes their ability to address scientific and healthcare related issues proposed in their various grants. PUBLIC HEALTH RELEVANCE: This request provides state of the art cell sorting instrumentation to support investigators active in various health and scientific related area of research. These investigators are recipients of peer reviewed funding from the NIH and NCI addressing a wide range of health care and scientific questions. To achieve success, many of these studies require the ability to isolate (sort) unique cells from mixed populations which the investigator will use to answer questions and test hypothesis proposed in their funding application.

描述（由申请人提供）：我们建议用四个激光器（405、488、561和640 nm）FACSARIA-II-II SORP Cell Sorter替换犹他大学健康科学中心现有的FACSVantage SE细胞分辨率。现有工具是在1994年通过NCRR资金购买的，尽管有机构资助的升级，但不再满足调查人员的需求。 Vantage仅限于6色排序，但是由于缺乏横梁补偿，通过溢出在技术上不切实际的6种颜色而溢出来减少某些荧光色素的有效分辨率。 Vantage只能进行双向分类，样品处理相对较慢（4K-10K细胞/秒，取决于样品），并且无法使用日益常见的红色荧光蛋白（例如M-Cherry）。目前，研究人员正在对校园的新流式细胞术分析仪进行6-8种颜色分析，这些分析仪无法在细胞分类的有利位置复制。许多研究人员，处理有限的样品材料，都想对三到四个亚群进行排序，这需要在有利的情况下进行两次顺序运行，从而损失了一些有限的样本和增加的时间。使用红色荧光蛋白作为记者的研究者完全无法对已经收集成像数据的细胞进行使用这些探针进行分类。最后，制造商将在2010年后不再支持Vantage，使服务和更换零件有问题。 Vantage位于一个核心实验室中，是校园内唯一的共享使用细胞分系员，服务于医疗中心，亨斯曼癌症研究所，Eccles Genetics Institute，Howard Hughes医学研究所，药房和本科生。分析和分类多种样品材料，从细菌，酵母和细胞器中的细胞裂解物到斑马鱼细胞，淋巴组织，人类肿瘤材料和众多细胞系。这些服务支持NIH资助的研究，涉及癌症，免疫学，细胞生物学，药物开发，药物提供和公共卫生。通过当前的仪器，我们不再能够提供许多研究人员寻求最先进的细胞分选技术，这反过来又阻碍了他们解决各种赠款中提出的科学和医疗保健相关问题的能力。 公共卫生相关性：该要求提供了最先进的细胞分类工具，以支持活跃于各种健康和科学研究领域的研究人员。这些调查人员是来自NIH和NCI的同行审查资金的接收者，他们解决了广泛的医疗保健和科学问题。为了取得成功，其中许多研究都需要从混合种群中隔离（排序）独特细胞的能力，研究人员将用来回答问题并测试其资金应用中提出的假设。