Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qToF) Mass Spectrometer

离子淌度光谱仪 - 四极杆飞行时间 (IMS-qToF) 质谱仪

• 批准号: 10630627
• 负责人: Mary Kay H Pflum
• 金额: $ 68.5万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630627
• 关键词: 14 year old; 17 year old; Address; Advisory Committees; Affect; Age; Antibodies; Area; Arts; Biomedical Research; Communities; Computer software; Dedications; Development; Funding; Individual; Institution; Laboratories; Liquid Chromatography; Mass Spectrum Analysis; Medical; Methods; Mission; Modernization; Pharmaceutical Preparations; Productivity; Protein Conformation; Proteins; Request for Applications; Research; Research Activity; Research Personnel; Research Project Grants; Research Support; Resolution; Science; Scientist; Spectrometry; Strategic Planning; Structure; Technology; Time; United States National Institutes of Health; Universities; adduct; biomarker discovery; college; innovation; instrument; ion mobility; mass spectrometer; multiple omics; prevent; programs; small molecule

Summary Biomedical research in the modern age has been revolutionized by the high resolution and high sensitivity mass analysis provided by state-of-the-art mass spectrometry (MS) instruments. The Lumigen Instrument Center (LIC) at Wayne State University has a mission to provide researchers at the university and in the regional area with reliable and diverse user-based MS analysis to elevate their research productivity. As part of the strategic plan of the Mass Spectrometry Laboratory in the LIC, the Mass Spectrometry Advisory Committee identified two current challenges for users. First, the substantial down time of a 17-year old Liquid Chromatography-Time-of-Flight (LC-TOF) instrument has prevented vital high resolution mass analysis, which has dramatically affected user research productivity. Second, the outdated hardware and software of a 14 year-old LTQ Orbitrap XL has stalled application to variety of projects. Finally, development of new methods to address the unique needs of individual users is limited due to the absence of new and powerful technologies, including Ion Mobility Spectrometry (IMS). To address these challenges, this application requests funds for an Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qTOF) Mass Spectrometer at Wayne State University. The instrument will significantly enhance research accomplishment in mass spectrometry (MS) on our campus. First, the IMS-qTOF will replace outdated and failing MS instruments for routine high-resolution analysis of small molecules and biomolecules. Second, the instrument will provide the only mass spectrometer in a shared facility on campus with IMS capability, which will enable NIH-funded researchers to expand and transform their research activities. The multiple capabilities of the instrument will include Top-Down and Bottom-Up Protein identification, protein adduct and conjugation characterization, protein conformational studies, biomarker discovery, drug-antibody characterization, accurate mass determination, and multi-omics workflows. The instrument will support the research programs of at least 19 users at Wayne State University and the regional area, which include chemists, biologists, environmental scientists, and medical researchers. The IMS-qTOF will be housed and maintained in the Lumigen Instrument Center (LIC), which has strong institution support from the University, the Office of the Vice President of Research, and the College of Liberal Arts and Science. The LIC has an exemplary record of instrument stewardship, with dedicated staff, facilities, and management to support the long-term use of the instrument. Importantly, the user-focused structure of the LIC will promote development of a unique array of new methods for each user’s individual research projects. The acquisition of an IMS-qToF is aligned with the long-term strategic plan of the Mass Spectrometry Laboratory at the LIC, which seeks to provide state-of-the-art and reliable MS instruments to implement innovative user-initiated projects at Wayne State University and local area community.

概括 现代的生物医学研究已通过高分辨率和高灵敏度彻底改变 由最先进的质谱法（MS）仪器提供的质量分析。 Lumigen仪器 韦恩州立大学的中心（LIC）的使命是为大学和大学提供研究人员 具有可靠和潜水用户的MS分析的区域区域，以提高其研究生产率。作为 LIC中质谱实验室战略计划的一部分，质谱咨询 委员会确定了当前对用户的挑战。首先，17岁的大量停机时间 液相色谱时间飞行时间（LC-TOF）仪器可以防止重要的高分辨率质量 分析，对用户研究生产率产生了巨大影响。其次，过时的硬件和 14岁的LTQ Orbitrap XL的软件已将应用程序应用于各种项目。最后，发展 由于缺乏新的和 功能强大的技术，包括离子迁移率光谱法（IMS）。 为了应对这些挑战，该应用程序要求资金用于离子移动光谱法 - 四极杆 韦恩州立大学的飞行时间（IMS-QTOF）质谱仪。该乐器将大大 增强我们校园中质谱（MS）的研究成就。首先，IMS-QTOF将 替换过时的和失败的MS仪器，以常规的小分子高分辨率分析和 生物分子。其次，该乐器将在校园的共享设施中提供唯一的质谱仪 具有IMS的能力，这将使NIH资助的研究人员能够扩展和改变他们的研究 活动。该乐器的多重功能将包括自上而下和自下而上的蛋白质 鉴定，蛋白质加合物和结合表征，蛋白质构象研究，生物标志物 发现，药物抗体表征，准确的质量测定和多摩学工作流。 仪器将支持韦恩州立大学至少19个用户的研究计划和 区域地区，包括化学家，生物学家，环境科学家和医学研究人员。 IMS-QTOF将被安置在Lumigen仪器中心（LIC）中，该中心很强 大学的机构支持，研究副校长办公室和学院 文科和科学。 LIC具有仪器管理的典范记录，并有专门的员工， 设施和管理层支持该工具的长期使用。重要的是，以用户为中心 LIC的结构将促进为每个用户的个人开发独特的新方法 研究项目。 IMS-QTOF的获取与群众的长期战略计划保持一致 LIC的光谱实验室，该实验室旨在提供最先进可靠的MS工具 在韦恩州立大学和当地社区实施创新的用户启动项目。