POST TRANSLATIONAL MODIFICATION OF RHOA

RHOA 的翻译后修饰

• 批准号: 8171309
• 负责人: Michael A Glotzer
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171309
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Cytokinesis; Funding; Gel; Grant; Institution; Mass Spectrum Analysis; Modification; Monomeric GTP-Binding Proteins; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; insight; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The small GTPase RhoA is a critical switch protein for cytokinesis. In a recent set of experiments, we found that ectopically expressed (and endogenous, to a lesser extent) RhoA is covalently modified and migrates with about a 4kd shift to a slower mobility form on a SDS gel. RhoA is a target for additional modifications and many of these have provided significant insight. We propose to characterize this modification by mass spectrometry.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 小GTPase RhoA是细胞因子的关键开关蛋白。在最近的一组实验中，我们发现异位表达（并且在较小程度上）RhoA是共价修改的，并以大约4KD的转移向SDS凝胶上的较慢的迁移率迁移。 Rhoa是进行其他修改的目标，其中许多提供了重要的见解。我们建议通过质谱法表征这种修饰。