IDENTIFICATION OF NOVEL MEMBERS OF S CEREVISIAE CYTOKINESIS APPARATUS

酿酒酵母细胞分裂装置新成员的鉴定

• 批准号: 8171447
• 负责人: DAVID G DRUBIN
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171447
• 关键词: Biological Assay; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytokinesis; Elements; Funding; Grant; Human; Institution; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; United States National Institutes of Health; Yeasts; member; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As important elements of human cytokinesis are highly conserved in S. cerevisiae, I am exploiting yeast's experimental advantages to study cytokinesis. To isolate cytokinetic ring contraction from related processes of the cell and give greater control of experimental parameters, I am developing a cell free functional assay for cytokinetic ring contraction. Excitingly, I have found it possible to isolate intact cytokinetic apparatus from yeast. To move to a definitive list of machinery sufficient for cytokinetic ring contraction, I am seeking to identify novel factors that are associated with partially purified, intact cerevisiae cytokinesis apparatus.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 由于人类胞质分裂的重要元素在酿酒酵母中高度保守，因此我正在利用酵母的实验优势来研究胞质分裂。 为了将细胞因子环收缩与细胞的相关过程分离并更好地控制实验参数，我正在开发一种用于细胞因子环收缩的无细胞功能测定。 令人兴奋的是，我发现可以从酵母中分离出完整的细胞因子装置。 为了获得足以使细胞分裂环收缩的机械的明确列表，我正在寻求识别与部分纯化的、完整的酿酒酵母胞质分裂装置相关的新因子。