CENTROSOME ASSEMBLY IN C ELEGANS EMBRYOS

线虫胚胎中的中心体组装

• 批准号: 8170844
• 负责人: THOMAS MUELLER-REICHERT
• 金额: $ 2.49万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170844
• 关键词: Caenorhabditis elegans; Cell Cycle; Cell Cycle Regulation; Cells; Centrioles; Centrosome; Cilia; Computer Retrieval of Information on Scientific Projects Database; Cytokinesis; Daughter; Embryo; Ensure; Flagella; Funding; Grant; Inherited; Institution; Light; Maintenance; Mediating; Microscopy; Microtubules; Mothers; Nature; Organelles; Organism; Pathway interactions; Play; Ploidies; Process; Proteins; RNA Interference; Research; Research Personnel; Resources; Role; Rotation; Signal Transduction; Source; Staging; Tube; United States National Institutes of Health; migration; restoration; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Centrioles are organelles that play essential roles in the formation of cilia and flagella, and they participate in cytokinesis, cell cycle control and centrosome organization. The cell's single centrosome, which is inherited as a spindle pole, duplicates only once per cell cycle, a process that is regulated through the control of centriole duplication. Centriole duplication is strictly controlled to ensure the formation of a bipolar spindle and the maintenance of ploidy. It has been known for many years that daughter centrioles assemble at right angles to the older, or "mother" centriole. The significance of this and the pathway for daughter centriole assemble remain mysterious. We are using the correlative light and EM tomography approach to study centrosome assembly in staged C. elegans embryos. Our initial results showed that daughter centriole assembly in this organism begins with the formation of a central tube, just as the pronuclei appear (Pelletier, L., O'Toole, E.T., Schwager, A., Hyman, A.A., and M¿ller-Reichert, T. (2006) Centriole assembly in Caenorhabditis elegans. Nature 444, 619-23). The central tube elongates during pronuclear migration, and the assembly of singlet microtubules on the tube occurs during pronuclear rotation. Using EM-tomography in combination with RNA interference and restoration microscopy we have found evidence suggesting that tube formation and elongation requires the SAS-5/SAS-6 proteins and an upstream signal mediated by SPD-2 and ZYG-1. We further show that although the assembly of the central tube still occurs, singlet microtubules fail to assemble in the absence of SAS-4, suggesting that it is required specifically at that step of assembly. The centriole assembly pathway we describe here in C. elegans may provide a framework for further studies on centriole assembly in other centriole bearing organisms.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 Centrioles是在纤毛和鞭毛的形成中起着重要作用的细胞器，它们参与了细胞因子，细胞周期控制和中心体组织。该细胞的单个中心体是遗传为主轴极的，每个细胞周期仅重复一次，该过程通过控制中心重复的控制。严格控制了Centriole重复，以确保形成双极纺锤体和维持倍倍。众所周知，女儿Centrioles与年龄较大或“母亲” Centriole以直角组装。这一点的意义和女儿Centriole组装的途径仍然神秘。我们正在使用矫正光和EM断层扫描方法来研究秀丽隐杆线虫胚胎中的中心体组装。我们的最初结果表明，这种生物体中的女子中心装配始于中央管的形成，就像前核外观一样（Pelletier，L.，O'Toole，E.T.，E.T.，Schwager，A.，Hyman，A.A。，A.A.中央管在前核迁移过程中拉长，并且在牙旋旋转过程中，导管上单个微管的组装发生。使用EM-Tograton与RNA干扰和恢复显微镜结合使用，我们发现了证据表明，管形成和伸长需要SAS-5/SAS-6蛋白以及由SPD-2和ZYG-1介导的上游信号。我们进一步表明，尽管中央管的组装仍在发生，但在没有SAS-4的情况下，单个微管无法组装，这表明在组装的那一步中需要特别需要它。我们在秀丽隐杆线虫中描述的中心装配途径可能为进一步研究其他中心轴承生物中的中心装配提供了一个框架。