Spatial and temporal control of Rho family GTPases

Rho 家族 GTPases 的空间和时间控制

• 批准号: 10622772
• 负责人: Michael A Glotzer
• 金额: $ 44.69万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10622772
• 关键词: Caenorhabditis elegans; Cell division; Cell membrane; Cell physiology; Cells; Congenital Abnormality; Drosophila genus; Family; Genetic; Guanosine Triphosphate Phosphohydrolases; Malignant Neoplasms; Molecular; Monomeric GTP-Binding Proteins; Morphogenesis; Nematoda; Process; Proteins; Role; Tissues; live cell imaging; model organism; optogenetics; polarized cell; rho

This proposal focuses on dissecting of the molecular mechanisms involved in the control of cell division, cell polarization, and morphogenesis. These simultaneously interrelated and functionally distinct processes are controlled by small GTPases, switch like proteins that primarily act at cell membranes to remodel cellular physiology. These proteins perform numerous functions in cells and tissues and their activity is highly regulated. We propose to study the role of several small GTPases during these processes in intact model organisms, including the nematode C. elegans and the fruit fly, Drosophila using a combination of live cell imaging, classical genetics, and optogenetics. We will also dissect the mechanisms responsible for the spatial and temporal activation of these GTPases which is critical for cell division, cell polarization, and morphogenesis. Given the high degree of conservation of these processes, information gained in this project could aid our understanding of certain cancers and the origin of some birth defects.

该提案的重点是剖析涉及的分子机制 同时控制细胞分裂、细胞极化和形态发生。 相互关联且功能不同的过程由小型 GTPase、开关控制 就像主要作用于细胞膜以重塑细胞生理学的蛋白质一样。 蛋白质在细胞和组织中执行多种功能，并且其活性非常高 我们建议研究几种小型 GTP 酶在这些过程中的作用。 完整模型生物中的过程，包括线虫秀丽隐杆线虫和果实 结合活细胞成像、经典遗传学和 我们还将剖析负责空间和光遗传学的机制。 这些 GTP 酶的暂时激活对于细胞分裂、细胞极化、 考虑到这些过程的高度保守性， 在这个项目中获得的信息可以帮助我们了解某些癌症和 一些出生缺陷的起源。

项目成果

Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation.

• DOI: 10.7554/elife.83992
• 发表时间: 2022-12-19
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Longhini, Katrina M.;Glotzer, Michael
• 通讯作者: Glotzer, Michael

Small GTPases modulate intrinsic and extrinsic forces that control epithelial folding in Drosophila embryos.

小 GTP 酶调节控制果蝇胚胎中上皮折叠的内在和外在力。

• DOI: 10.1080/21541248.2021.1926879
• 发表时间: 2021
• 期刊: Small GTPases
• 作者: Rich,Ashley;Glotzer,Michael
• 通讯作者: Glotzer,Michael