POST TRANSLATIONAL MODIFICATION OF RHOA

RHOA 的翻译后修饰

• 批准号: 7957748
• 负责人: Michael A Glotzer
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957748
• 关键词: Biology; Computer Retrieval of Information on Scientific Projects Database; Cytokinesis; Funding; Fungal Genome; Gel; Grant; Institution; Mass Spectrum Analysis; Modification; Monomeric GTP-Binding Proteins; Post-Translational Protein Processing; Proteins; RHOA gene; Research; Research Personnel; Resources; Source; United States National Institutes of Health; insight; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The small GTPase RhoA is a critical switch protein for cytokinesis. In a recent set of experiments, we found that ectopically expressed (and endogenous, to a lesser extent) RhoA is covalently modified and migrates with about a 4kd shift to a slower mobility form on a SDS gel. RhoA is a target for additional modifications and many of these have provided significant insight. We propose to characterize this modification by mass spectrometry.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 小 GTP 酶 RhoA 是胞质分裂的关键开关蛋白。在最近的一组实验中，我们发现异位表达（在较小程度上是内源性）的 RhoA 被共价修饰，并在 SDS 凝胶上以大约 4kd 的位移迁移到较慢的迁移形式。 RhoA 是额外修改的目标，其中许多修改提供了重要的见解。我们建议通过质谱来表征这种修饰。