IDENTIFICATION OF PROTEIN COMPLEXES AND PHOSPHORYLATION SITES OF PROTEINS

蛋白质复合物和蛋白质磷酸化位点的鉴定

• 批准号: 8171261
• 负责人: DANNEL MCCOLLUM
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171261
• 关键词: Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytokinesis; Fission Yeast; Funding; Grant; Homologous Gene; Human; Institution; Lead; Mass Spectrum Analysis; Mitosis; Pathway interactions; Phosphorylation; Phosphorylation Site; Phosphotransferases; Play; Proteins; Regulation; Research; Research Personnel; Resources; Role; Signal Pathway; Site; Source; Tumor Suppression; United States National Institutes of Health; Yeasts; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The McCollum lab studies a signaling pathway in the fission yeast S. pombe, termed the septation initiation network (SIN), which functions at the end of mitosis to trigger initiation of cytokinesis. Most of the components of the SIN pathway have been identified, however their precise molecular interactions and the role phosphorylation plays in their regulation have not been determined. We have been using mass spectrometry to characterize molecular interactions among SIN components and identify sites of regulatory phosphorylation. These studies have focused on the Sid2-Mob1 kinase complex and a positive (Clp1) and negative regulator (Dma1) of the SIN. All of the components of this pathway are conserved in humans and function in cytokinesis and in tumor suppression. We have initiated studies to identify proteins that interact with the human homolog of Sid2, called Lats2. These studies should lead to a more comprehensive understanding of the SIN pathway in yeast, which we hope will help in the characterization of tumor suppression pathways in humans.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 McCollum实验室研究了裂变酵母菌中的信号传导途径，称为Septation Initiation Network（SIN），该网络在有丝分裂结束时起作用，以触发细胞因子的引发。已经鉴定出了SIN途径的大多数成分，但是尚未确定它们的精确分子相互作用和在调节中的作用磷酸化作用。 我们一直在使用质谱法来表征SIN成分之间的分子相互作用并确定调节磷酸化的位点。 这些研究集中在SID2-MOB1激酶复合物以及SIN的阳性（CLP1）和阴性调节剂（DMA1）上。该途径的所有组成部分在人类中均保守，在细胞因子和肿瘤抑制中的功能。我们已经开始研究，以鉴定与SID2人类同源物相互作用的蛋白质，称为LATS2。 这些研究应该导致对酵母中的罪恶途径的更全面的了解，我们希望这将有助于表征人类的肿瘤抑制途径。