TRANSGENIC & GENOTYPING CORE

转基因

• 批准号: 8360681
• 负责人: Melissa Larson
• 金额: $ 15万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360681
• 关键词: Cells; Centers of Research Excellence; Core Facility; Cryopreservation; Development; Disease model; Electroporation; Embryo; Equipment; Funding; Gene Targeting; Gene-Modified; Genotype; Grant; Injection of therapeutic agent; Laboratories; Molecular; Mus; National Center for Research Resources; Principal Investigator; Production; Regulation; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Services; Source; Techniques; Time; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; blastocyst; cost; embryonic stem cell; gene function; sperm cell; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Gene-modified mice have served as models of disease and tools for investigative research into the function of genes for the past 30 years. Despite their widespread use, the techniques and equipment required to produce these mice remain specialized. The Transgenic and Gene-Targeting Facility, therefore, supports the research of COBRE investigators by creating gene-modified mice and providing embryo services. The Core generates transgenic mice by pronuclear injection, chimeric mice by injection of blastocysts and targeted embryonic stem cells by electroporation, as well as providing support services such as genotyping and sperm cryopreservation. A centralized core facility such as ours negates the need for each laboratory to expend the time and money required to become proficient in embryo handling and transgenic mouse production, thereby accelerating research.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 过去 30 年来，基因修饰小鼠一直充当疾病模型和基因功能调查研究的工具。 尽管它们被广泛使用，但生产这些小鼠所需的技术和设备仍然是专业化的。 因此，转基因和基因靶向设施通过培育基因修饰小鼠并提供胚胎服务来支持 COBRE 研究人员的研究。 Core 通过原核注射产生转基因小鼠，通过注射胚泡和电穿孔定向胚胎干细胞产生嵌合小鼠，并提供基因分型和精子冷冻保存等支持服务。 像我们这样的集中式核心设施使每个实验室无需花费时间和金钱来精通胚胎处理和转基因小鼠生产，从而加速研究。