KANSAS U COBRE: TRANSGENIC & GENOTYPING CORE

堪萨斯大学 COBRE：转基因

基本信息

批准号：
7959570
负责人：
Melissa Larson
金额：
$ 14.82万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2010-06-30
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The gene-modified mouse has become a standard and indispensable tool for investigating the function of genes in vivo. Although the modified mouse is common, the techniques employed for its generation require skill and specialized equipment. It is far more cost- and time-effective to centralize both the equipment and skill into a core, shared, research support facility, as has been done here, rather than equipping and training individual laboratories. By centralizing operations, the gene-modified mouse is available as a research tool to all investigators. The Transgenic and Gene-Targeting Facility provides services to COBRE investigators that include generation of transgenic mice by pronuclear injection, generation of chimeric mice by blastocyst injection, targeting of embryonic stem cells by electroporation, cryopreservation of sperm from gene-modified mice and genotyping of gene-modified mouse litters. All of these services have been requested by and performed for members of the COBRE.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此，可以在其他清晰的条目中表示。列出的机构是对于中心，这不一定是调查员的机构。基因修饰的小鼠已成为研究体内基因功能的标准和必不可少的工具。尽管修改后的鼠标很常见，但为其生成的技术需要技巧和专业设备。像在这里所做的那样，将设备和技能集中到核心，共享的研究支持设施中要有成本和时间效率要高得多，而不是为单个实验室装备和培训单个实验室。通过集中操作，可以将基因修饰的小鼠作为研究工具提供给所有研究人员。转基因和基因靶向设施为毛cre研究者提供服务，包括通过前核注射来产生转基因小鼠，通过胚泡注射来产生嵌合小鼠，通过电穿孔靶向胚胎干细胞，通过电穿孔来靶向胚胎干细胞，从基因改性小鼠和基因的基因分型的胚胎保存， - 修饰的鼠标垃圾。所有这些服务均已由COBRE成员要求并执行。