FLOW CYTOMETRY CORE

流式细胞术核心

• 批准号: 8168494
• 负责人: MARK S DOONER
• 金额: $ 25.43万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168494
• 关键词: Cell Separation; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Computer software; Deposition; Development; Flow Cytometry; Funding; Grant; Head; Housing; Institution; Names; Phenotype; Population; Research; Research Personnel; Resources; Rhodamine; Rhodamines; Sorting - Cell Movement; Source; Stem cells; Techniques; Time; United States National Institutes of Health; Work; experience; instrument; progenitor; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Core will provide expertise in hematopoetic stem cell separations, microvesicle sorting and cell phenotyping analysis. The Core will be headed by Mr. Mark Dooner. Mr. Dooner is an experienced flow cytometrist and will devote 50% time to this Core. Mr. Dooner will be the dedicated operator and devote 100% of his time to the Core. A To Be Named Technician will be able to provide backup and cross coverage for the Core. We have decided to have an accomplished full time technician solely dedicated to this COBRE, since this is justified by the complexity and extent of the proposed work. This Core features a new InFlux highspeed cell sorter from Cytopeia Inc. This sorter is capable of carrying out stem cell separations, single cell deposition and is housed within a biosafety cabinet. This Core is housed in a dedicated room. The facility is equipped with a new LSRII (BD instruments) with Facs Diva software. Investigators will work with the core and prepare their cell populations for sorting or analysis. The Core will routinely separate Lineage negative Rhodamine lo Hoechst lo (LRH), LT-HSC, ST-HSC, CLP, CMP and Lineage negative Sca+ stem/progenitor populations. The core will also sort and characterize microvesicles and analyze populations for lineage profiles. The core will serve all projects in this COBRE carrying out both separations and analysis work. The Core also remains dedicated to the development of new separation techniques and the enhancement of known stem cell and microvesicle populations.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 流式细胞术核心将提供造血干细胞分离、微泡分选和细胞表型分析方面的专业知识。 核心团队将由 Mark Dooner 先生领导。 Dooner 先生是一位经验丰富的流式细胞仪专家，将投入 50% 的时间在该核心上。 Dooner 先生将成为专职操作员，并将 100% 的时间投入到核心上。 待指定的技术人员将能够为核心提供备份和交叉覆盖。我们决定聘请一名出色的全职技术人员专门负责此 COBRE，因为拟议工作的复杂性和范围证明这是合理的。 该核心配备了 Cytopeia Inc. 的新型 InFlux 高速细胞分选机。该分选机能够进行干细胞分离、单细胞沉积，并安装在生物安全柜内。 该核心位于一个专用房间内。该设施配备了带有 Facs Diva 软件的新型 LSRII（BD 仪器）。 研究人员将与核心合作并准备其细胞群以进行分类或分析。 Core 将常规分离谱系阴性罗丹明 lo Hoechst lo (LRH)、LT-HSC、ST-HSC、CLP、CMP 和谱系阴性 Sca+ 干细胞/祖细胞群。 该核心还将对微泡进行分类和表征，并分析群体的谱系谱。 该核心将为 COBRE 中的所有项目提供服务，执行分离和分析工作。 该核心还致力于开发新的分离技术以及增强已知的干细胞和微泡群体。