Omics, Bioinformatics and Flow Cytometry Core

组学、生物信息学和流式细胞术核心

• 批准号: 10630580
• 负责人: MICHAEL R GARRETT
• 金额: $ 22.41万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630580
• 关键词: African American population; Apoptosis; Bioinformatics; Biological; Biological Assay; Biological Process; Cardiorenal syndrome; Cell Cycle; Cell Nucleus; Cell Proliferation; Cell Separation; Cells; Centers of Research Excellence; Clinical; Complement; Consultations; Country; Data Set; Disease; Enhancement Technology; Ensure; Equipment; Faculty; Faculty Workshop; Flow Cytometry; Funding; Gene Expression; Generations; Genetic; Genomics; Goals; Health; High Prevalence; Historically Black Colleges and Universities; Immune; Infrastructure; Institution; Instruction; Maintenance; Mass Spectrum Analysis; Metabolic Diseases; Methodology; Mississippi; Molecular; Molecular Analysis; Molecular Biology; Morbidity - disease rate; Names; Nucleic Acids; Obesity; Phase; Phenotype; Pilot Projects; Population; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resources; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Sorting; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training and Education; United States; Work; XCL1 gene; cell type; complex data; epigenome; genome sequencing; genomic data; glucose uptake; improved; insight; instrument; instrumentation; meetings; mortality; multidisciplinary; multiple omics; next generation sequencing; programs; release of sequestered calcium ion into cytoplasm; service delivery; single-cell RNA sequencing; training opportunity; transcriptome; transcriptome sequencing; transcriptomics; undergraduate student; whole genome

CORE C – OMICS, BIOINFORMATICS AND FLOW CYTOMETRY CORE PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phases 1 and 2, the CMDRC - Core C provided COBRE investigators and external researchers access to technical expertise and equipment to perform a wide variety of molecular, genetic, and next generation sequencing studies. During Phases 1 and 2, Core C also made major advances toward ensuring sustainability of its services. During Phase 3, the newly named Core C - Omics, Bioinformatics and Flow Cytometry will extend its services and offer cutting-edge cellular and molecular analysis through building on existing infrastructure supported in Phases 1 and 2 (Genomics and Mass Spectrometry) to offer new cutting-edge omics methodologies as well as offer new capabilities through recently acquired state-of-the-art cell sorting and flow cytometry equipment. The Omics and Bioinformatics Sub-Core and Cell Sorting/Flow Cytometry Sub-Core will allow CMDRC investigators comprehensive molecular or systems biology approaches to their research questions through detailed insight into cell-type specific function, generation of “omics” datasets, and ultimately integration with detailed phenotype information to provide greater depth and insight into biological processes associated with obesity, cardiorenal, and metabolic diseases. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge omics services, including next generation sequencing technologies (single cell RNA sequencing, spatial transcriptomics) and facilitate proteomic technologies by leveraging existing IDeA funded infrastructure (offer and coordinate proteomics services and through IDeA National Resource for Quantitative Proteomics program); (2) to provide CMDRC investigators access to cutting-edge cell sorting and flow cytometry expertise and services; (3) to provide education and training opportunities for students, trainees, and faculty in omics technologies and flow cytometry; and (4) to provide continuous improvement in existing assays and services and to enhance technological capabilities through acquisition of new cutting- edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC - Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art cell sorting, flow cytometry, and omics technologies to generate greater insights into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase 3 will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)) access to equipment and expertise that would otherwise not be available in our region of the country.

核心C - OMICS，生物信息学和流式细胞仪核心 项目摘要/摘要 心脏和代谢性疾病研究中心（CMDRC）的目标是将 一个基本，临床和人群科学家组成的多学科小组，以协同为主题 心脏和代谢性疾病。在第1阶段和第2阶段，CMDRC -Core C提供了毛绒 调查人员和外部研究人员可以使用技术专长和设备来执行各种各样 分子，遗传和下一代测序研究。在第1阶段和第2阶段，Core C也制成 确保其服务可持续性的重大进展。在第3阶段，新命名的Core C- OMICS，生物信息学和流式细胞仪将扩展其服务，并提供最先进的蜂窝蜂窝 通过在第1阶段和第2阶段支持的现有基础设施上建立分子分析（基因组学和 质谱法）提供新的尖端法律方法，并通过 最近获得的最先进的细胞分类和流式细胞仪设备。幻象和生物信息学 子核和细胞分选/流式细胞仪子核将允许CMDRC研究者全面 分子或系统生物学方法通过详细了解细胞类型来解决其研究问题 特定功能，“ OMICS”数据集的生成，以及最终与详细表型集成 信息以更深入和了解与肥胖，心脏，心脏相关的生物学过程 和代谢疾病。 Core C有四个目标：（1）为CMDRC调查人员提供技术访问 专业知识和尖端的OMICS服务，包括下一代测序技术（单个单元格 RNA测序，空间转录组学）并通过利用现有思想来促进蛋白质组学技术 资助的基础设施（要约和协调蛋白质组学服务以及通过Ideas National资源 定量蛋白质组学计划）； （2）为CMDRC调查人员访问尖端细胞分类 以及流式细胞仪专业知识和服务； （3）为学生提供教育和培训机会， 学员和教师教学学院技术和流式细胞仪； （4）提供持续的改进 现有的测定和服务，并通过获得新的剪裁来增强技术能力 - 边缘仪器将有助于核心的可持续性。因此，这是CMDRC的目标 - 核心C帮助研究人员推进肥胖，心脏和代谢疾病的科学发现 通过应用最先进的细胞分类，流式细胞术和OMICS技术来生成更大的 对这些疾病的见解。除了CMDRC的调查人员，阶段还继续资助了鞋垫 3将对提供外部研究人员产生重大影响（在密西西比州的其他本科 机构和历史上的黑人学院和大学（HBCUS））访问设备和专业知识 否则在我们该国地区将不可用。