Core C - Genomics, Animal Models and Advanced Phenotyping Core

核心 C - 基因组学、动物模型和高级表型分析核心

• 批准号: 10403631
• 负责人: MICHAEL R GARRETT
• 金额: $ 38.62万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-05 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403631
• 关键词: Animal Model; Animals; Biological Assay; Biological Process; Breeding; Cardiovascular Models; Cardiovascular system; Cells; Centers of Research Excellence; Clinical; Collection; Complement; Consultations; Country; Custom; Data Set; Disease; Ensure; Equipment; Faculty; Faculty Workshop; Fee-for-Service Plans; Funding; Gene Expression; Generations; Genes; Genetic Engineering; Genomics; Genotype; Goals; Grant; Health; High Prevalence; Historically Black Colleges and Universities; Inbred Mouse; Inbreeding; Infrastructure; Institution; Instruction; Kidney; Kidney Diseases; Knockout Mice; Letters; Maintenance; Measurement; Medical center; Metabolic Diseases; Methodology; Methylation; Mississippi; Modeling; Molecular; Molecular Genetics; Morbidity - disease rate; Obesity; Phase; Phenotype; Physiological; Population; Preparation; Proteins; Publications; Rattus; Research; Research Personnel; Research Project Grants; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training; Training and Education; Transgenic Mice; Transgenic Organisms; United States; United States National Institutes of Health; Universities; Work; animal model development; base; bioinformatics tool; epigenetic regulation; epigenome; exome sequencing; genome sequencing; genomic data; improved; insight; instrumentation; meetings; metabolic phenotype; microbial; mortality; mouse model; multidisciplinary; next generation sequencing; novel; service delivery; training opportunity; transcriptome; transcriptome sequencing; undergraduate student; whole genome

CORE C – GENOMICS, ANIMAL MODELS AND ADVANCED PHENOTYPING PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phase 1, CMDRC-Core C provided COBRE investigators and external researchers' access to technical expertise and equipment to perform a wide variety of molecular, genetic, microarray and gene expression studies that would otherwise not be possible. Core C also provided investigators at UMMC access to novel transgenic and knockout mouse and rat models of cardiovascular renal and metabolic disease that are only available at UMMC. In total, CMDRC-Core C completed several hundred genomic studies as well as generated thousands of animals for detailed phenotyping resulting in numerous publications and several NIH R01 funded grants. In Phase II, CMDRC-Core C major project, pilot grants, and external researchers will have access to state-of-the-art genomic technologies through the Genomics Sub-Core and large collection of animal models (inbred, transgenic, and gene-edited rat and mouse models) as well as advanced phenotyping methodologies through the Animal Models and Advanced Phenotyping Sub-Core. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge genomic services, including next generation sequencing technologies (RNA sequencing, 16S microbial sequencing, custom amplicon panels, whole genome sequencing, and exome sequencing); (2) to provide CMDRC investigators access to large collection of unique animal models of obesity, cardiorenal and metabolic diseases, along with training in advanced phenotyping techniques; (3) to provide education and training opportunities for students, trainees, and faculty in genomics technologies and advanced animal phenotyping; and (4) to provide continuous improvement in existing assays, services, and enhance technological capabilities through acquisition of new cutting-edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC-Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art genomic technologies, specialized animal models, and advanced physiological measurements to generate greater insight into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase II will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)] access to equipment and expertise that would otherwise not be available in our region of the country.

核心C - 基因组学，动物模型和先进的表型 项目摘要/摘要 心脏和代谢性疾病研究中心（CMDRC）的目标是将 一个基本，临床和人群科学家组成的多学科小组，以协同为主题 心脏和代谢性疾病。在第1阶段，CMDRC核C提供了毛to研究者和 外部研究人员获得技术专长和设备的访问，以执行各种分子 否则不可能的遗传，微阵列和基因表达研究。核心也提供了 UMMC访问新型转基因和敲除鼠标的研究人员以及心血管的大鼠模型 仅在UMMC上可用的肾脏和代谢疾病。 CMDRC核C总共完成了几个 数百种基因组研究以及生成数千只动物进行详细的表型，导致 许多出版物和几项NIH R01资助的赠款。在第二阶段，CMDRC核C大项目，飞行员 赠款，外部研究人员将可以通过 基因组学亚核和大量动物模型（近交，转基因和基因编辑的大鼠，以及 小鼠模型）以及通过动物模型和先进的高级表型方法 表型子核。 Core C有四个目标：（1）为CMDRC调查人员提供技术访问 专业知识和尖端基因组服务，包括下一代测序技术（RNA 测序，16S微生物测序，自定义扩增子面板，整个基因组测序和外显子组 测序）; （2）为CMDRC调查人员提供大量独特动物模型的访问 肥胖，心脏和代谢疾病，以及晚期表型技术的训练； （3）到 为基因组技术的学生，学员和教师提供教育和培训机会， 先进的动物表型； （4）为现有测定，服务和 通过获取新的尖端仪器来增强技术能力，这将有助于 核心的可持续性。因此，CMDRC核C的目的是帮助调查人员前进 肥胖，心脏和代谢性疾病的科学发现通过最先进 基因组技术，专业动物模型和高级物理测量以生成 对这些疾病的洞察力更大。除了CMDRC调查人员，在 第二阶段将对提供外部研究人员产生重大影响（在其他密西西比州本科 机构和历史上的黑人学院和大学（HBCUS）]获得设备和专业知识 否则在我们该国地区将不可用。