IMAGING CORE

成像核心

• 批准号: 7610571
• 负责人: MARK S DOONER
• 金额: $ 15.72万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610571
• 关键词: Cell Transplantation; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Geographic Locations; Grant; Green Fluorescent Proteins; Image; Immunohistochemistry; Institution; Marrow; Microscopy; Morphology; Nature; Research; Research Personnel; Resources; Source; Standards of Weights and Measures; Suspension substance; Suspensions; Tissues; United States National Institutes of Health; beta-Galactosidase; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core will focus on identifying the nature of cells after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of male DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers will be confirmed using confocal or deconvolution fluorescent microscopy.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心将集中于识别骨髓细胞移植后细胞的性质。供体细胞及其特定谱系将通过共焦和反向卷积荧光显微镜进行确定和共定位。通过表达GFP，β-半乳糖苷酶或雄性DNA的存在，将在组织切片或细胞悬浮液中跟踪供体细胞。标准形态，地理位置和免疫组织化学将确定已确定的供体细胞的谱系。供体和谱系标记的共定位将使用共焦或反向卷积荧光显微镜确认。