PRIMATE PLURIPOTENT CELLS FOR AUTOLOGOUS TRANSPLANTATION

用于自体移植的灵长类多能细胞

• 批准号: 8173333
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173333
• 关键词: Adult; Autologous Transplantation; Cells; Chimera organism; Computer Retrieval of Information on Scientific Projects Database; Embryo; Fibroblasts; Funding; Grant; Institution; Macaca mulatta; Monkeys; Primates; Research; Research Personnel; Resources; Skin; Somatic Cell; Source; Testing; United States National Institutes of Health; Validation; embryonic stem cell; induced pluripotent stem cell; pluripotency; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this proposal is to investigate the potential of rhesus monkey pluripotent cells experimentally induced by reprogramming of adult somatic cells using two alternative approaches: somatic cell nuclear transfer (SCNT) and direct reprogramming. In this application, we will generate genetically identical SCNT-ES and iPS cells from adult monkey skin fibroblasts and compare them to each other and to ES cells produced from fertilized embryos using an array of extensive tests including the ability to generate chimeras, the ultimate validation of their pluripotency.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该提案的目的是研究通过使用两种替代方法重编程成人体细胞通过重新编码成年细胞来实验诱导的恒河猴多能细胞的潜力：体细胞核转移（SCNT）和直接重编程。在此应用中，我们将使用一系列广泛的测试（包括产生嵌合体的能力），对成年猴子皮肤成纤维细胞产生遗传相同的SCNT-ES和IPS细胞，并将它们相互比较，并与受精胚胎产生的ES细胞进行比较。