Flow Cytometry Core

流式细胞术核心

• 批准号: 9356956
• 负责人: MARK S DOONER
• 金额: $ 30.61万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9356956
• 关键词: Biological Sciences; Biotechnology; Cell Aging; Cell Cycle; Cell Separation; Cells; Centers of Research Excellence; Charge; Communities; Computer software; Consultations; Data Analyses; Data Collection; Deposition; Development; Experimental Designs; Fee-for-Service Plans; Flow Cytometry; Hematopoietic stem cells; Hospitals; Manuscripts; Phase; Phenotype; Pilot Projects; Population; Preparation; Research; Research Personnel; Resources; Rhode Island; Services; Sorting - Cell Movement; Speed; Stem cells; Techniques; Universities; Work; experience; instrument; instrumentation; laser cell sorter; operation; relating to nervous system

Flow Cytometry Core Abstract The Flow Cytometry Core will provide expertise in stem cell separations, cell sorting and cell phenotyping analysis. The Core will be co-directed by Dr. Loren Fast and Mr. Mark Dooner. Mr. Dooner is an experienced flow cytometrist with sorting and analysis of neural and hematopoietic stem cells, and will oversee the daily operation including cell sorting and analysis. Dr. Fast will serve as an advisor and resource to the investigators for experimental design, data interpretation and manuscript preparation. During Phase I, instrument upgrades created a five laser cell sorter that is unique in the Rhode Island research community. This Core features an Influx high speed cell sorter (BD Biosciences). This sorter is capable of carrying out stem cell separations, single cell deposition, and simultaneous six-way sorting and is housed within a biosafety cabinet. This Core is housed in a newly constructed and dedicated suite. The facility is equipped with a LSRII flow cytometer (BD Biosciences) with FACS Diva software for cell cycle analysis and routine flow cytometry analysis. Investigators will work with the Core and prepare their cell populations for sorting or analysis. The Core will serve all projects in this COBRE carrying out both separations and analysis work free of charge. Non-COBRE investigators are making use of the Core on a fee for service basis however COBRE Projects will be given priority. The Core remains dedicated to the development of new separation techniques and the enhancement of known neural and hematopoietic stem cell populations.

流式细胞术核心 抽象的 流式细胞术核心将提供干细胞分离、细胞分选和细胞表型分析方面的专业知识 分析。 The Core 将由 Loren Fast 博士和 Mark Dooner 先生共同指导。杜纳先生是一位经验丰富的 流式细胞仪对神经干细胞和造血干细胞进行分选和分析，并将监督日常检查 操作包括细胞分选和分析。法斯特博士将担任研究人员的顾问和资源 用于实验设计、数据解释和手稿准备。第一阶段，仪器升级 创建了五激光细胞分选机，这在罗德岛研究界是独一无二的。该核心具有 Influx 高速细胞分选仪（BD Biosciences）。该分选机能够进行干细胞分离， 单细胞沉积和同步六向分选，并安装在生物安全柜内。这个核心是 位于新建的专用套房内。该设施配备了LSRII流式细胞仪（BD Biosciences）与 FACS Diva 软件进行细胞周期分析和常规流式细胞术分析。调查员 将与 Core 合作并准备其细胞群以进行分类或分析。核心将为所有人服务 COBRE 中的项目免费进行分离和分析工作。非COBRE 调查人员正在按服务收费使用 Core，但将提供 COBRE 项目 优先事项。核心仍然致力于开发新的分离技术和增强 已知的神经和造血干细胞群。