Transgenic and Gener-Targeting Core

转基因和基因靶向核心

基本信息

批准号：
8534216
负责人：
Melissa Larson
金额：
$ 15.99万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

Core B: Transgenic and Gene Targeting Core The Transgenic and Gene Targeting Core integrates with and enhances the capabilities of our exisitng Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This Core will assist COBRE investigators by providing cost-efffective serviecs for the generation, characterization, and maintenance of genetic mouse models. Ofthe 32 senior scientists in the Center, 9 are frequent users ofthe Core, and 2 of the 5 COBRE Beginning Investigator projects also have plans to utilize this facility. This Core will provide COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient females to create transgenic mice. In addition, this Core will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The Core also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for Beginning Investigators and Center members and KUMC will continue to provide significant institutional support for this facility. The Facility is staffed by a Technical Director, Scientific Director, and two Research Associates, who will interface with two COBRE co-advisors

核心 B：转基因和基因靶向核心转基因和基因打靶核心与我们现有的转基因和基因打靶设施集成并增强了其能力，在创建转基因和基因敲除小鼠方面拥有良好的成功记录。该核心将通过为遗传小鼠模型的生成、表征和维护提供具有成本效益的服务来协助 COBRE 研究人员。该中心的 32 名高级科学家中，有 9 名是 Core 的频繁用户，5 个 COBRE 初级研究员项目中的 2 个也有计划利用该设施。该核心将为 COBRE 科学家提供将 DNA 显微注射到受精卵中并将胚胎移植到受体雌性体内以培育转基因小鼠的服务。此外，该核心还将提供嵌合小鼠的创建服务，包括多种ES细胞系的维持、ES细胞的电穿孔和选择、核型测定以及所选ES细胞的囊胚注射。科布雷资金将用于扩大这些服务，包括用于显微注射或基因靶向的 DNA 操作和制备，以及转基因和基因敲除小鼠的基因分型。 Core 还提供小鼠品系的冷冻保存。 COBRE 基金将为初级研究者和中心成员提供服务补贴，KUMC 将继续为该设施提供重要的机构支持。该设施配备了一名技术总监、科学总监和两名研究助理，他们将与与两名 COBRE 联合顾问