KANSAS U COBRE TRANSGENIC & GENOTYPING CORE

堪萨斯 U COBRE 转基因

• 批准号: 7721032
• 负责人: Melissa Larson
• 金额: $ 16.15万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721032
• 关键词: Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Equipment; Funding; Gene Targeting; Generations; Genotype; Grant; Injection of therapeutic agent; Institution; Kansas; Microinjections; Modeling; Mus; Polymerase Chain Reaction; Range; Research; Research Personnel; Research Support; Resources; Services; Source; Techniques; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; blastocyst; design; embryonic stem cell; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic and Gene-Targeting Facility provides many research support services to COBRE investigators and was therefore added as a Core Facility. Services include the generation of transgenic mice by pronuclear injection, gene targeting in embryonic stem cells, generation of chimeric mice by blastocyst microinjection and genotyping by Polymerase Chain Reaction. Because both the equipment and expertise required to perform these services is costly, establishing a Core Facility enables all COBRE investigators access to these techniques and subsequent research models. The Transgenic Facility has also been instrumental in designing experiments for COBRE investigators, and has expanded the range of services offered to investigators via funding from the COBRE.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 转基因和基因靶向设施为 COBRE 研究人员提供许多研究支持服务，因此被添加为核心设施。 服务包括通过原核注射产生转基因小鼠、胚胎干细胞中的基因靶向、通过囊胚显微注射产生嵌合小鼠以及通过聚合酶链反应进行基因分型。 由于执行这些服务所需的设备和专业知识都很昂贵，因此建立核心设施使所有 COBRE 研究人员能够获得这些技术和后续研究模型。 转基因设施在为 COBRE 研究人员设计实验方面也发挥了重要作用，并通过 COBRE 的资助扩大了为研究人员提供的服务范围。