Transgenic and Gener-Targeting Core

转基因和基因靶向核心

基本信息

批准号：
8691924
负责人：
Melissa Larson
金额：
$ 16.44万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

Core B: Transgenic and Gene Targeting Core The Transgenic and Gene Targeting Core integrates with and enhances the capabilities of our exisitng Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This Core will assist COBRE investigators by providing cost-efffective serviecs for the generation, characterization, and maintenance of genetic mouse models. Ofthe 32 senior scientists in the Center, 9 are frequent users ofthe Core, and 2 of the 5 COBRE Beginning Investigator projects also have plans to utilize this facility. This Core will provide COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient females to create transgenic mice. In addition, this Core will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The Core also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for Beginning Investigators and Center members and KUMC will continue to provide significant institutional support for this facility. The Facility is staffed by a Technical Director, Scientific Director, and two Research Associates, who will interface with two COBRE co-advisors.

核心B：转基因和基因靶向核心转基因和基因靶向核心与我们的Exisitng转基因和基因靶向设施的能力融合并增强了成功的良好记录，以创建转基因和敲除小鼠的成功记录。该核心将通过为遗传小鼠模型的生成，表征和维护提供成本效能的服务来帮助毛cre研究者。在中心的32位高级科学家中，有9位是核心的使用者，而5个柯布雷初学者项目中有2个也有计划利用此设施。该核心将为毛病科学家提供对受精卵的微型DNA的服务，并将胚胎转移到受体雌性中，以产生转基因小鼠。此外，该核心将提供创建嵌合小鼠的服务，包括维持几种ES细胞系，电动孔和选择ES细胞的选择，核型的测定和胚泡注射所选的ES细胞。鞋垫资金将用于扩展这些服务，以包括用于微分注射或基因靶向的DNA的操纵和制备，以及转基因和基因敲除小鼠的基因分型。核心还提供小鼠线的冷冻保存。 COBRE资金将为初学者和中心成员提供补贴服务，KUMC将继续为该设施提供大量的机构支持。该设施由技术主管，科学总监和两名研究助理组成，他们将与两个Cobre共同顾问交互。