Neuroimaging and Electrophysiology Facility (NIEF)

神经影像和电生理学设施 (NIEF)

基本信息

批准号：
10628976
负责人：
JORGE David MIRANDA
金额：
$ 43.35万
依托单位：
UNIVERSITY OF PUERTO RICO MED SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10628976
关键词：
Academia Academic degree Anesthesia procedures Animals Applications Grants Area Biological Biophysics Brain Calcium Caribbean region Cells Centers of Research Excellence Collaborations Communities Computer software Core Facility Cultured Cells Data Data Analyses Development Educational workshop Electrophysiology (science)Equipment Faculty Fee-for-Service Plans Fluorescence Microscopy Funding Generations Goals Grant Human Resources Image Industry Institution Island Laboratories Legal patent Manuscripts Microscope Microscopy Mission Molecular Monitor Neurobiology Neuronal Plasticity Oocytes Outcome Participant Peer Review Phase Postdoctoral Fellow Privatization Procedures Productivity Publications Publishing Puerto Rico Research Research Infrastructure Research Personnel Research Project Grants Scanning Science Scientist Services Slice Students Techniques Technology Training Universities Visit data acquisition environmental enrichment for laboratory animals experience experimental study faculty support graduate student innovation instrument instrumentation interest investigator training neuroimaging new technology research facility role model service providers tool two-photon ultra high resolution undergraduate student voltage

Academia Academic degree Anesthesia procedures Animals Applications Grants Area Biological Biophysics Brain Calcium Caribbean region Cells Centers of Research Excellence Collaborations Communities Computer software Core Facility Cultured Cells Data Data Analyses Development Educational workshop Electrophysiology (science)Equipment Faculty Fee-for-Service Plans Fluorescence Microscopy Funding Generations Goals Grant Human Resources Image Industry Institution Island Laboratories Legal patent Manuscripts Microscope Microscopy Mission Molecular Monitor Neurobiology Neuronal Plasticity Oocytes Outcome Participant Peer Review Phase Postdoctoral Fellow Privatization Procedures Productivity Publications Publishing Puerto Rico Research Research Infrastructure Research Personnel Research Project Grants Scanning Science Scientist Services Slice Students Techniques Technology Training Universities Visit data acquisition environmental enrichment for laboratory animals experience experimental study faculty support graduate student innovation instrument instrumentation interest investigator training neuroimaging new technology research facility role model service providers tool two-photon ultra high resolution undergraduate student voltage

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项目摘要

Project Summary: The Neuroimaging and Electrophysiology Facility (NIEF) was established to increase the competitiveness of scientists in Puerto Rico via the incorporation of microscopy and electrophysiology techniques in their research projects. This aim was achieved by the establishment of two research cores, one at the Molecular Science Research Center (MSRC) and another at the Institute of Neurobiology (INB), from the University of Puerto Rico. Both facilities support a diversified group of investigators on the island. During phases 1 and 2 of COBRE, the NIEF acquired state-of-the-art instruments that are expensive and not accessible in most laboratories, departments and/or institutions. Among the equipment obtained or supervised by the NIEF are confocal, two-photon, and super resolution microscopes. Also available are electrophysiology rigs for oocytes, cultured cells, brain slice, and anesthetized animals to monitor cell electrical activity. As a result of the NIEF research infrastructure and expert technical personnel, 113 peer reviewed manuscripts have been published, leading to 1,816 citations. Moreover, data obtained from the NIEF cores were included in 84 grant proposals that were submitted to federal, state, and private agencies, and from those submitted, 48 were funded. Furthermore, research performed in the NIEF permitted the innovation of new technology by supporting investigators with the development of 7 patents. Finally, over 50 graduate students from eight different departments and three main research institutions have benefitted from the NIEF cores by being able to complete at least one of their theses aims and attain their academic degrees, thus demonstrating the impact of the NIEF in the scientific community of Puerto Rico. These results confirm the beneficial impact of the NIEF cores on the investigators in the island. Therefore, in this COBRE phase 3 proposal, the goal is to maintain, strengthen, and support the NIEF cores by implementing three aims: 1) to provide scientists in Puerto Rico with state-of-the-art instrumentation in microscopy and electrophysiology, 2) to support the investigators in the island with expert staff that will help them with the acquisition and analysis of the data from microscopy and electrophysiology techniques, and 3) to train researchers in several technologies associated with these two approaches (microscopy and electrophysiology) via seminars, workshop, and hands-on training. Thus, the facilities and services will continue to help investigators use the most innovative electrophysiological and microscopy technology to generate interdisciplinary projects to study biological and biophysical phenomena under the guidance of experienced and excellent staff. Fulfillment of these aims will enable a transition of the NIEF to a completely self-sustained facility beyond COBRE phase 3 and a role model for Puerto Rico and the Caribbean.

项目摘要：建立了神经影像学和电生理学设施（NIEF）以提高竞争力通过将显微镜和电生理技术纳入波多黎各的科学家研究项目。这一目标是通过建立两个研究核心来实现的，一个是在分子上科学研究中心（MSRC）和来自大学神经生物学研究所（INB）的另一个波多黎各。这两个设施都支持岛上多元化的调查人员。在阶段1中还有2个柯布尔（Cobre 大多数实验室，部门和/或机构。在获得或监督的设备中惩罚是共聚焦，两光子和超级分辨率显微镜。还可以使用电生理学用于卵母细胞，培养细胞，脑切片和麻醉动物的钻机以监测细胞电活动。作为这是NIEF研究基础设施和专家技术人员的结果，113个同行审查了手稿已发表，导致1,816次引用。此外，从锥形核心获得的数据是包括提交给联邦，州和私人机构的84项赠款提案，从已提交，有48名资助。此外，在疼痛中进行的研究允许新的创新通过为研究人员提供7项专利的支持，技术。最后，超过50个毕业生来自八个不同部门和三个主要研究机构的学生从中受益通过能够完成至少一个论文的目标并获得学位，刺激核心，从而证明了疼痛在波多黎各科学界的影响。这些结果确认疼痛核心对岛上调查人员的有益影响。因此，在这个毛绒中第三阶段提案，目标是通过实施三个目的：1）为波多黎各的科学家提供显微镜和电生理学，2）支持岛上的调查人员，专家工作人员将帮助他们从显微镜和电生理技术中获取和分析数据，以及3）训练与这两种方法相关的几种技术研究人员（显微镜和电生理学）通过研讨会，研讨会和动手培训。因此，设施和服务将继续帮助调查人员使用最具创新性的电生理和显微镜技术来在研究生物学和生物物理现象的指导下生成跨学科项目以研究生物和生物物理现象经验丰富且出色的员工。实现这些目标将使疼痛过渡到一个完全自我维持的设施超出了毛cr阶段第3阶段，以及波多黎各和加勒比海。