Two-Photon Microscope Patch Clamp Integrated System
双光子显微镜膜片钳集成系统
基本信息
- 批准号:6877299
- 负责人:
- 金额:$ 49.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-04-01 至 2008-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): A two-photon scanning confocal microscope is requested for integration with a patch clamp setup. This integrated system will be used for experiments that combine imaging with simultaneous electrophysiological recording from living cells. Six investigators, Ed Chapman, Roberto Coronado, Robert Fettiplace, Meyer Jackson, Jeff Walker, and Lea Ziskind-Conhaim form a core of major users of this system. All of these investigators have expertise in both electrophysiology and imaging, and all have similar needs for a system that can combine laser scanning microscopy and patch clamping. They will investigate various aspects of signaling in cardiac myocytes, hair cells, presynaptic terminals, and neuroendocrine terminals. Calcium plays vital roles in the physiology of each of these systems. Most of the experiments to be conducted primarily with calcium imaging to obtain high resolution information about calcium signals in real time as cells are subject to electrical stimulation. Each of the six investigators has NIH funding for research that can be dramatically enhanced by this instrument. Among other advantages, this system will enable Chapman to investigate the regulation of fusion pores by synaptotagmin, Coronado to investigate Ca 2+dynamics at individual Ca 2+release sites, Fettiplace to study Ca 2+ in individual stereocilia, Jackson to investigate spatial variations in Ca 2+dynamics in nerve terminals, Walker to apply caged signaling molecules to T-tubules and Ziskind-Conhaim to investigate the role of specific cell types in spinal rhythmicity. The shared instrument grant will enhance all of these research programs by providing better signal-to-noise in Ca 2+ imaging, better tissue penetration in imaging experiments in slices, and better localization in caged compound photolysis.
描述(由申请人提供):需要双光子扫描共焦显微镜与膜片钳装置集成。该集成系统将用于将成像与活细胞同步电生理记录相结合的实验。 Ed Chapman、Roberto Coronado、Robert Fettiplace、Meyer Jackson、Jeff Walker 和 Lea Ziskind-Conhaim 六名调查员构成了该系统主要用户的核心。所有这些研究人员都拥有电生理学和成像方面的专业知识,并且都对能够将激光扫描显微镜和膜片钳结合起来的系统有类似的需求。他们将研究心肌细胞、毛细胞、突触前末梢和神经内分泌末梢信号传导的各个方面。钙在每个系统的生理学中都起着至关重要的作用。大多数实验主要通过钙成像进行,以便在细胞受到电刺激时实时获取有关钙信号的高分辨率信息。六名研究人员中的每一位都获得了 NIH 的研究资助,该仪器可以极大地增强研究效果。除其他优点外,该系统将使 Chapman 能够研究突触结合蛋白对融合孔的调节,Coronado 能够研究单个 Ca 2+ 释放位点的 Ca 2+ 动力学,Fettiplace 能够研究单个静纤毛中的 Ca 2+,Jackson 能够研究单个静纤毛中的 Ca 2+ 空间变化。神经末梢的 Ca 2+ 动力学,Walker 将笼状信号分子应用于 T 管,Ziskind-Conhaim 研究特定细胞类型在脊髓节律中的作用。共享仪器拨款将通过在 Ca 2+ 成像中提供更好的信噪比、在切片成像实验中提供更好的组织穿透性以及在笼式复合光解中更好的定位来增强所有这些研究项目。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MEYER B. JACKSON其他文献
MEYER B. JACKSON的其他文献
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{{ truncateString('MEYER B. JACKSON', 18)}}的其他基金
Fusion pores in endocrine and synaptic exocytosis
内分泌和突触胞吐作用中的融合孔
- 批准号:
10449673 - 财政年份:2022
- 资助金额:
$ 49.99万 - 项目类别:
Fusion pores in endocrine and synaptic exocytosis
内分泌和突触胞吐作用中的融合孔
- 批准号:
10615868 - 财政年份:2022
- 资助金额:
$ 49.99万 - 项目类别:
Integration of Experience-Induced Gene Expression and Circuit Functions
经验诱导的基因表达和电路功能的整合
- 批准号:
10404503 - 财政年份:2018
- 资助金额:
$ 49.99万 - 项目类别:
Integration of Experience-Induced Gene Expression and Circuit Functions
经验诱导的基因表达和电路功能的整合
- 批准号:
10132411 - 财政年份:2018
- 资助金额:
$ 49.99万 - 项目类别:
Integration of Experience-Induced Gene Expression and Circuit Functions
经验诱导的基因表达和电路功能的整合
- 批准号:
9897551 - 财政年份:2018
- 资助金额:
$ 49.99万 - 项目类别:
Ca2+ buffering in the regulation of secretion from peptidergic nerve terminals
肽能神经末梢分泌调节中的 Ca2 缓冲
- 批准号:
10000213 - 财政年份:2017
- 资助金额:
$ 49.99万 - 项目类别:
Ca2+ buffering in the regulation of secretion from peptidergic nerve terminals
肽能神经末梢分泌调节中的 Ca2 缓冲
- 批准号:
10240521 - 财政年份:2017
- 资助金额:
$ 49.99万 - 项目类别:
Circuit Mechanisms of Information Processing and Storage in Brain Slices
脑切片信息处理和存储的电路机制
- 批准号:
9320901 - 财政年份:2015
- 资助金额:
$ 49.99万 - 项目类别:
Transgenic Mice for Hybrid Voltage Sensor Imaging of Neural Circuitry
用于神经回路混合电压传感器成像的转基因小鼠
- 批准号:
8675971 - 财政年份:2013
- 资助金额:
$ 49.99万 - 项目类别:
Summer research experience for undergraduates in neuroscience
神经科学本科生暑期研究经历
- 批准号:
8794487 - 财政年份:2013
- 资助金额:
$ 49.99万 - 项目类别: