Confocal Microscope for Core Imaging Facility

用于岩心成像设施的共焦显微镜

• 批准号: 6877361
• 负责人: Chi-Bin Chien
• 金额: $ 44.67万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877361
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): Imaging of biological specimens has become an increasingly important tool in biomedical science, with the abilities to image live specimens and multiple fluorophores being especially important. We propose to purchase a Zeiss LSM510 confocal microscope system to be housed in the Cell Imaging Facility of the University of Utah School of Medicine. This confocal would replace an obsolete entry-level confocal (Olympus FVX) and improve the capabilities of the facility by adding a wide choice of excitation wavelengths, as well as fast switching between multiple emission filters. An environmentally controlled specimen chamber and motorized X-Y stage would particularly facilitate live-cell imaging experiments. No instrument with this set of features is currently available anywhere at the University of Utah. The core Cell Imaging Facility is centrally located in the School of Medicine, and is overseen by a fulltime Ph.D. director with postdoctoral experience. Under his direction the facility has been highly successful, with heavy use of the existing confocal microscopes (>35 hours/week/microscope). The director provides training and support to users in addition to maintaining the microscopes and administering their use. Furthermore, every year he teaches a didactic microscopy course together with the Principal Investigator. There is a strong institutional commitment to providing core microscopy facilities. The University of Utah fully funds the facility director's salary, and guarantees the cost of service contracts. The instrument's use would be monitored by an oversight committee, which would liaise with a committee that oversees all of the School of Medicine's core facilities. This instrument would primarily serve 7 major users from 4 different departments in the School of Medicine. These researchers are funded by 14 current R01, P01 or R37 NIH grants and are studying systems including cultured cells, C. elegans, and zebrafish. All of these projects involve live-cell imaging, and would benefit significantly from the improved flexibility in excitation and emission wavelengths offered by the proposed instrument.

描述（由申请人提供）：生物样本成像已成为生物医学中日益重要的工具，对活体样本和多种荧光团进行成像的能力尤其重要。我们建议购买蔡司 LSM510 共焦显微镜系统，安装在犹他大学医学院的细胞成像设施中。这种共焦将取代过时的入门级共焦（奥林巴斯 FVX），并通过增加多种激发波长选择以及多个发射滤光片之间的快速切换来提高设备的功能。环境控制的样品室和电动 X-Y 平台将特别有利于活细胞成像实验。目前，犹他大学的任何地方都没有具有这组功能的仪器。 核心细胞成像设施位于医学院中心，由全职博士负责监督。主任具有博士后经历。在他的指导下，该设施取得了巨大成功，大量使用了现有的共焦显微镜（> 35 小时/周/显微镜）。除了维护显微镜和管理其使用之外，主任还为用户提供培训和支持。此外，每年他都会与首席研究员一起教授显微镜教学课程。有一个强有力的机构承诺提供核心显微镜设施。犹他大学全额资助设施主管的工资，并保证服务合同的费用。 该仪器的使用将由一个监督委员会进行监督，该委员会将与负责监督医学院所有核心设施的委员会进行联络。 该仪器主要服务于医学院4个不同院系的7个主要用户。这些研究人员由 14 项当前的 R01、P01 或 R37 NIH 拨款资助，正在研究包括培养细胞、线虫和斑马鱼在内的系统。所有这些项目都涉及活细胞成像，并将大大受益于激发和发射波长的灵活性的提高 拟议的文书。