Leica TCS SP2 AOBS UV Confocal System

Leica TCS SP2 AOBS 紫外共焦系统

• 批准号: 6876961
• 负责人: Ivan Julian Dmochowski
• 金额: $ 49.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876961
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): Advances in biomedical research funded by the NIH in our School of Arts and Sciences (SAS) and Engineering School require ready access to a state-of-the-art confocal laser scanning microscope. Numerous currently supported projects within Chemistry, Physics, Biology, and Bioengineering at the University of Pennsylvania would benefit from this instrument. These include in vivo studies of polymersomes and related polymeric "worms" (Bioengineering); incorporating multiple porphyrin-based fluorophores into such vesicles, and localizing molecular beacons within cells (Chemistry); fate mapping zebrafish development through ultraviolet uncaging of fluorophores and small molecules (Biology); and investigating soft materials, neuronal interactions, and photodynamic therapy (Physics). The assembled user group possesses considerable expertise in optical imaging techniques, and, in particular, confocal laser scanning microscopy (CLSM). Routine access to CLSM is crucial for validating the localization of polymeric assemblies, proteins, and drugs within living cells and animals. CLSM provides hundreds-of-nanometer resolution images of complex 3-dimensional structures, and allows the interrogation of molecules with light, either within living systems or soft materials. Recent advances in commercially available confocal microscopes place these instruments increasingly in the domain of Chemistry and Biophysics. The current lack of a confocal microscope within the Chemistry Department, and the shortage of general-access, state-of- the-art CLSM facilities available to physical scientists at Penn negatively impacts studies important to many NIH-supported programs. An urgent need for access to these new technologies and a few shared scientific goals have brought together investigators from four different departments for this proposal.

描述（由申请人提供）：由 NIH 资助的艺术与科学学院 (SAS) 和工程学院的生物医学研究进展需要随时使用最先进的共焦激光扫描显微镜。宾夕法尼亚大学化学、物理、生物学和生物工程领域目前支持的许多项目都将从该仪器中受益。这些包括聚合物囊泡和相关聚合物“蠕虫”的体内研究（生物工程）；将多个基于卟啉的荧光团掺入此类囊泡中，并在细胞内定位分子信标（化学）；通过荧光团和小分子的紫外线释放来绘制斑马鱼发育的命运图（生物学）；并研究软材料、神经元相互作用和光动力疗法（物理学）。聚集的用户组在光学成像技术，特别是共焦激光扫描显微镜（CLSM）方面拥有丰富的专业知识。常规获取 CLSM 对于验证活细胞和动物内聚合物组件、蛋白质和药物的定位至关重要。 CLSM 提供复杂 3 维结构的数百纳米分辨率图像，并允许用光询问生命系统或软材料内的分子。商用共聚焦显微镜的最新进展使这些仪器越来越多地应用于化学和生物物理学领域。目前化学系内缺乏共焦显微镜，而且宾夕法尼亚大学物理科学家缺乏通用的、最先进的 CLSM 设施，这对许多 NIH 支持的项目的重要研究产生了负面影响。由于迫切需要获得这些新技术和一些共同的科学目标，来自四个不同部门的研究人员聚集在一起提出了这项提案。

项目成果

Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue.

对活体组织中空间限定的单细胞进行体内转录组分析 (TIVA)。

• 发表时间: 2014-02
• 影响因子: 48
• 作者: Lovatt, Ditte;Ruble, Brittani K;Lee, Jaehee;Dueck, Hannah;Kim, Tae Kyung;Fisher, Stephen;Francis, Chantal;Spaethling, Jennifer M;Wolf, John A;Grady, M Sean;Ulyanova, Alexandra V;Yeldell, Sean B;Griepenburg, Julianne C;Buckley, Peter T;Kim, J
• 通讯作者: Kim, J

Ruthenium-induced allylcarbamate cleavage in living cells.

• DOI: 10.1002/anie.200601752
• 发表时间: 2006-08-25
• 期刊: Angewandte Chemie
• 作者: Craig Streu;E. Meggers
• 通讯作者: E. Meggers

Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos.

用于斑马鱼胚胎中 let-7 miRNA 双向光调制的笼状寡核苷酸。

• DOI: 10.1016/j.bmc.2013.04.082
• 发表时间: 2013-10-15
• 期刊: Bioorganic & medicinal chemistry
• 影响因子: 3.5
• 作者: Griepenburg, Julianne C.;Ruble, Brittani K.;Dmochowski, Ivan J.
• 通讯作者: Dmochowski, Ivan J.

Ruthenium-Caged Antisense Morpholinos for Regulating Gene Expression in Zebrafish Embryos.

用于调节斑马鱼胚胎基因表达的钌笼反义吗啡啉。

• 发表时间: 2015-04-01
• 影响因子: 8.4
• 作者: Griepenburg, J C;Rapp, T L;Carroll, P J;Eberwine, J;Dmochowski, I J
• 通讯作者: Dmochowski, I J

Caging metal ions with visible light-responsive nanopolymersomes.

用可见光响应纳米聚合物囊泡捕获金属离子。

• 发表时间: 2015-01-20
• 作者: Griepenburg, Julianne C;Sood, Nimil;Vargo, Kevin B;Williams, Dewight;Rawson, Jeff;Therien, Michael J;Hammer, Daniel A;Dmochowski, Ivan J
• 通讯作者: Dmochowski, Ivan J