Zeiss LSM 510 META Confocal Microscope

蔡司 LSM 510 META 共焦显微镜

• 批准号: 6877461
• 负责人: STANLEY C FROEHNER
• 金额: $ 46.19万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877461
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy; electrophysiology; fluorescence resonance energy transfer; protein structure function

DESCRIPTION (provided by applicant): This proposal requests support for a confocal microscope with state of the art features for multi-fluorescent protein studies, FRET imaging and electrophysiological studies. The Zeiss LCS 510 META confocal microscope will provide technology that is currently unavailable to and is pivotal for the research effort of eleven independent laboratories in the biomedical sciences. Research programs in all eleven laboratories are now at a critical phase where long term, reliable, and frequent access to this equipment is essential for further progress. All investigators are NIH-funded with research programs that include the following areas of investigation: mechanisms of ion channel and transporter function, synaptogenesis, structure and function of opioid receptors, signal transduction pathways in early embryonic development, calcium signaling in cardiac and vascular smooth muscle, the regulation of expression and localization of muscarinic acetylcholine receptors, stem cell repair of cardiac infarcts, bacterial pathogenesis and metabolism and cellular actions of cannabinoids in neurons. Although the research in these laboratories is primarily basic science in nature, it also has major and direct implications for numerous human diseases and maladies, including cardiac and vascular disease, cancer, muscular dystrophy, bacterial pathogenesis, defects of the visual system and drug addiction and abuse. The core investigators represent five separate departments in two schools. The broad range of projects proposed by the primary user group is representative of a larger group of potential users of the new microscope system. The requested instrument would be placed in the established Keck Imaging Facility located on the University of Washington campus and would be made available to other investigators when it is not being used by the core group of researchers. The operation and management of this equipment, the internal advisory committee and institutional support for the confocal microscope are described. The Keck Imaging Center and its director, Greg Martin, are widely known and valued at the University of Washington and its affiliated institutions, thus assuring that the new instrument and its state-of-the- art capabilities will have a broadly based clientele.

描述（由申请人提供）：该提案要求支持具有最先进功能的共聚焦显微镜，用于多荧光蛋白研究、FRET 成像和电生理学研究。蔡司 LCS 510 META 共焦显微镜将为生物医学科学领域 11 个独立实验室的研究工作提供目前尚无法实现且至关重要的技术。所有十一个实验室的研究项目目前都处于关键阶段，长期、可靠和频繁地使用这些设备对于取得进一步进展至关重要。所有研究人员均由 NIH 资助，研究项目包括以下研究领域：离子通道和转运蛋白功能的机制、突触发生、阿片受体的结构和功能、早期胚胎发育中的信号转导途径、心脏和血管平滑肌中的钙信号传导、毒蕈碱乙酰胆碱受体表达和定位的调节、心肌梗塞的干细胞修复、细菌发病机制和神经元中大麻素的代谢和细胞作用。尽管这些实验室的研究本质上主要是基础科学，但它也对许多人类疾病和疾病具有重大和直接的影响，包括心脏和血管疾病、癌症、肌肉萎缩症、细菌发病机制、视觉系统缺陷和药物成瘾和虐待。核心调查人员代表两所学校的五个不同部门。主要用户组提出的广泛项目代表了新显微镜系统的更大的潜在用户组。所需的仪器将放置在华盛顿大学校园内已建立的凯克成像设施中，并在核心研究人员小组不使用时提供给其他研究人员。描述了该设备的操作和管理、内部咨询委员会以及共焦显微镜的机构支持。凯克成像中心及其主任 Greg Martin 在华盛顿大学及其附属机构中广为人知并受到重视，从而确保了新仪器及其最先进的功能将拥有广泛的客户群。