LASER SCANNING CONFOCAL MICROSCOPE

激光扫描共焦显微镜

• 批准号: 6877422
• 负责人: NIGEL W BUNNETT
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-09 至 2007-05-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877422
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; computer program /software; confocal scanning microscopy; protein quantitation /detection

DESCRIPTION (provided by applicant): This proposal is for the purchase of a Zeiss LSM 510 confocal microscope to be used by seven principal investigators and their laboratories in support of eighteen NIH funded projects. These groups will occupy approximately 85% time on the instrument, minor users will have access to the equipment at other times. The requested instrument offers the flexibility of a multi-user microscope that will be the "work horse" for many laboratories. A four-laser microscope is requested (blue diode 405 nm, argon 458, 477, 488 and 514 nm, Hene 543 nm, Hene 633 nm lasers), allowing simultaneous or sequential observation of commonly used blue yellow green, red and far red emitting fluorophores. The instrument will be mounted on a Zeiss 200 Axiovert inverted microscope, equipped with a heated stage motorized in the x, y and z axes. This microscope will provide the flexibility of observation of fixed tissues and cultured cells, as well as observations of dynamic processes in living cells. Objectives are requested to permit the optimal collection of fluorescence signals from fixed tissues and cells, and of cultured cells at an oil/glass/water interface. Software is requested that will permit three-dimensional reconstruction of objects, real time analysis of signal intensities, and sequential collection of z-series from multiple cells in different fields for prolonged periods due to the x/y motorized stage). All of the major users are experienced microscopists, most of whom have previously used confocal microscopy. Existing confocal microscopes in the investigators' laboratories are coming to the end of their useful life and presently require considerable and costly maintenance. Importantly, service contracts will not be offered in the future, requiring availability of a new instrument. Other instruments at UCSF are extensively used by other groups, are not sufficiently flexible for the required uses, or are at other distant campuses. Some of these instruments will be moved to other campuses when faculty relocates to the new UCSF campus at Mission Bay. An impartial faculty committee will oversee use of the microscope. A recharge system will be implemented to fund the routine maintenance and support of the microscope. All users will take a classroom based and hands on training program. Institutional support includes provision of newly refurbished space, funding of technical support for three years, provision of computing support, and fiscal and administrative oversight and support.

描述（由申请人提供）：本提案旨在购买一台 Zeiss LSM 510 共焦显微镜，供 7 位主要研究人员及其实验室使用，以支持 18 个 NIH 资助的项目。这些群体将占据仪器上大约 85% 的时间，少数用户可以在其他时间访问设备。所需的仪器提供了多用户显微镜的灵活性，这将成为许多实验室的“主力”。需要四激光显微镜（蓝色二极管 405 nm，氩气 458、477、488 和 514 nm，氦烯 543 nm，氦烯 633 nm 激光器），允许同时或顺序观察常用的蓝黄绿光、红光和远红光发射荧光团。该仪器将安装在 Zeiss 200 Axiovert 倒置显微镜上，配备在 x、y 和 z 轴上电动加热的载物台。该显微镜将提供观察固定组织和培养细胞的灵活性，以及​​观察活细胞动态过程的能力。要求物镜能够最佳地收集固定组织和细胞以及油/玻璃/水界面处培养细胞的荧光信号。 要求软件能够实现物体的三维重建、信号强度的实时分析以及由于 x/y 电动平台而长时间从不同视野中的多个细胞连续收集 z 系列。所有主要用户都是经验丰富的显微镜专家，其中大多数人以前使用过共焦显微镜。研究人员实验室中现有的共焦显微镜的使用寿命即将结束，目前需要大量且昂贵的维护。重要的是，将来不会提供服务合同，需要提供新仪器。加州大学旧金山分校的其他仪器被其他团体广泛使用，对于所需用途而言不够灵活，或者位于其他遥远的校园。当教师搬迁到位于 Mission Bay 的 UCSF 新校区时，其中一些仪器将被转移到其他校区。一个公正的教职委员会将监督显微镜的使用。将实施充电系统来资助显微镜的日常维护和支持。所有用户都将参加基于课堂的实践培训计划。机构支持包括提供新装修的空间、三年技术支持资金、提供计算支持以及财政和行政监督和支持。