LIFTETIME MEASUREMENTS OF CRYPTOPHANE-XENON COMPLEXES

隐烷-氙复合物的寿命测量

• 批准号: 7955447
• 负责人: Ivan Julian Dmochowski
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955447
• 关键词: Affinity; Binding; Biosensor; Chemicals; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence; Funding; Goals; Grant; Institution; Laboratories; Magnetic Resonance Imaging; Measurement; Measures; Nature; Optics; Process; Relaxation; Research; Research Personnel; Resources; Screening for cancer; Solutions; Source; Therapeutic Intervention; Time; United States National Institutes of Health; Xenon; aqueous; chromophore; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our overall project is to develop 129-Xe MRI biosensors for early cancer detection and possible therapeutic intervention. We are currently synthesizing cryptophanes and other carriers that will have greater affinity for xenon, longer 129Xe hyperpolarized spin-lattice relaxation times, and unique chemical shifts. Cryptophane-A, an organic cage compound, binds xenon (Xe) in its internal cavity with the highest affinity of any known molecule and fluoresces with a maximum intensity around 314nm. The presence of excess Xe in aqueous solution with the cryptophane quenches the observed fluorescence with an efficiency of about 50% as measured by steady-state experiments. This quenching is believed to be largely static in nature, a result of the bound Xe atom residing very close to the veratole chromophores of the cryptophane. Fluorescence lifetime measurements would allow for the determination of the biomolecular quenching constant or a confirmation of the static quenching process.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们整个项目的目标是开发 129-Xe MRI 生物传感器，用于早期癌症检测和可能的治疗干预。我们目前正在合成 Cryptophane 和其他载体，它们对氙具有更大的亲和力、更长的 129Xe 超极化自旋晶格弛豫时间以及独特的化学位移。 Cryptophane-A 是一种有机笼状化合物，在其内腔中以任何已知分子中最高的亲和力结合氙 (Xe)，并在 314 nm 附近发出最大强度的荧光。水溶液中过量 Xe 与 Cryptophane 的存在会猝灭观察到的荧光，根据稳态实验测量，效率约为 50%。这种猝灭被认为本质上是静态的，这是由于结合的Xe原子非常靠近cryptophane的藜芦生色团而导致的。荧光寿命测量可以确定生物分子猝灭常数或确认静态猝灭过程。