Transgenic Mice for Hybrid Voltage Sensor Imaging of Neural Circuitry

用于神经回路混合电压传感器成像的转基因小鼠

基本信息

批准号：
8675971
负责人：
MEYER B. JACKSON
金额：
$ 22.15万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-15 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8675971
关键词：
Action Potentials Animals Axon Biological Neural Networks Brain Brain region Cell Count Cells Charge DNA Data Dendrites Development Electroporation Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Functional disorder Genetic Techniques Green Fluorescent Proteins Growth Associated Protein 43 Hybrids Image Laboratories Learning Lipid Bilayers Maps Membrane Membrane Potentials Memory Methods Movement Mus N-terminal Neurologic Neurons Neurosciences Research Optics Pattern Perception Performance Population Proteins Reporter Reporting Research Personnel Signal Transduction Slice Stimulus Techniques Testing Transgenic Mice Transgenic Organisms Work base brain cell cell type dipicrylamine improved in utero interest neural circuit neuronal cell body patch clamp promoter public health relevance relating to nervous system research study sensor tool voltage

项目摘要

DESCRIPTION (provided by applicant): The hybrid voltage sensor (hVOS) technique enables researchers to use green fluorescent protein and other fluorescent proteins to image membrane potential. Because hVOS probes are encoded by DNA, genetic techniques can be used to target them to specific types of cells. This project will develop mice with hVOS probes targeted to specific types of neurons in the mouse brain. Animals will be developed with hVOS probes expressed in a wide variety of brain regions and cell types. Animals will also be developed that can be used to target very specific populations of cells. Slices of brain prepared from these animals will be very useful in imaging experiments. Essentially any brain region and any nerve cell type will be accessible to studies using the strategy advanced in this work. With these animals researchers will be able to study the electrical activity of large numbers of cells simultaneously. A hVOS probe developed in this laboratory was found to preferentially target axons. Animals expressing this hVOS probe will be useful in general studies of axonal excitability.

描述（由申请人提供）：混合电压传感器（hVOS）技术使研究人员能够使用绿色荧光蛋白和其他荧光蛋白对膜电位进行成像。由于 hVOS 探针由 DNA 编码，因此可以使用遗传技术将其靶向特定类型的细胞。该项目将开发带有 hVOS 探针的小鼠，该探针针对小鼠大脑中特定类型的神经元。动物将使用在多种大脑区域和细胞类型中表达的 hVOS 探针进行开发。还将开发出可用于针对非常特定的细胞群的动物。从这些动物身上制备的大脑切片对于成像实验非常有用。基本上任何大脑区域和任何神经细胞类型都可以使用这项工作中先进的策略进行研究。通过这些动物，研究人员将能够同时研究大量细胞的电活动。该实验室开发的 hVOS 探针被发现优先瞄准轴突。表达这种 hVOS 探针的动物将有助于轴突兴奋性的一般研究。