Ultra High-Speed Imaging System

超高速成像系统

• 批准号: 6578577
• 负责人: PEI ZHONG
• 金额: $ 38.07万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6578577
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; charge coupled device camera; digital imaging; high throughput technology

DESCRIPTION (provided by applicant): The purpose of this shared instrumentation grant application is to acquire an Imacon 200 ultra high-speed imaging system to be shared by a group of NIH funded investigators at Duke University. The Major Users Group is supported by 7 R01 and 1 P01 grants from 3 different NIH institutes. These investigators (from the Depts. of Mechanical Engineering and Materials Science, Urologic Surgery, Cell Biology, Radiation Oncology, and Biomedical Engineering) have critical needs for high-speed imaging analyses in order to effectively advance their research. The projects that will immediately benefit from this proposed instrument cover a wide range of topics, from shock wave lithotripsy (SWL) for the treatment of kidney stone disease, to wound healing in SWL, to the design of liposomal drug carriers, to regulation of germ line stem cell division in Drosophila, to gene therapy for cancer treatment. The Imacon 200 offers a unique high-speed imaging system utilizing multiples of intensified CCD modules to provide simultaneous framing and streak recording, up to 16 frames of high-resolution images in each sequence. Each intensified CCD module can be gated electronically with an exposure time from 100 ms down to 5 ns, thus providing an effective framing rate from 10 frames/second to 200 million frames/second. The operation of the Imacon 200 system is controlled remotely via fiber optical link by a dedicated computer for reliable image capture and quantitative data extraction. No comparable equipment capable of performing any of these functions currently exists at Duke University. This system, therefore, is critical for performing high-speed photoelastic and shadowgraph imaging to better understand shock wave-stone interaction, intraluminal bubble dynamics in blood vessels, the role of bubble collapse and associated microstreaming in triggered release of liposomal carriers, microjet-faciliated gene delivery to Drosophila embryos, hyperthermia-mediated gene delivery and activation, DNA transport across the plasma and nuclei membranes of the cells during electroporation, and rheology of red blood cells in tumor microcirculation. The establishment of a core facility in ultra high-speed imaging will greatly enhance these research programs and the scientific contributions by these and other investigators at Duke University.

描述（由申请人提供）： 此共享仪器赠款申请的目的是获取iMacon 200超高成像系统，该系统将由杜克大学的NIH资助的研究人员共享。主要用户组由3个不同的NIH研究所的7 R01和1个P01赠款支持。这些研究者（来自机械工程和材料科学，泌尿外科手术，细胞生物学，放射肿瘤学和生物医学工程），对高速成像分析有至关重要的需求，以有效地提高其研究。将立即受益于该拟议仪器的项目涵盖了各种各样的主题，从冲击波碎石术（SWL）用于治疗肾脏石材疾病，到SWL中的愈合，再到脂质体药物载体的设计，再到果蝇在果蝇中的生殖线干细胞分裂的调节，再到基因治疗癌症治疗的基因治疗。 Imacon 200提供了一个独特的高速成像系统，利用加强的CCD模块的倍数提供同时的框架和条纹记录，每个序列中最多可达16帧的高分辨率图像。每个加强的CCD模块可以通过以电子方式向下曝光时间从100 ms下降到5 ns，从而提供从10帧/秒到2亿帧/秒的有效框架速率。 iMacon 200系统的操作通过专用计算机通过光纤链路进行远程控制，以可靠的图像捕获和定量数据提取。杜克大学目前没有能够执行任何这些功能的可比设备。 This system, therefore, is critical for performing high-speed photoelastic and shadowgraph imaging to better understand shock wave-stone interaction, intraluminal bubble dynamics in blood vessels, the role of bubble collapse and associated microstreaming in triggered release of liposomal carriers, microjet-faciliated gene delivery to Drosophila embryos, hyperthermia-mediated gene delivery and activation, DNA transport across the plasma and电穿孔过程中细胞的核膜和肿瘤微循环中红细胞的流变学。在超高成像中建立核心设施将极大地增强这些研究计划以及杜克大学和其他研究人员的科学贡献。