TRANSMISSION ELECTRON MICROSCOPE

透射电子显微镜

• 批准号: 6287986
• 负责人: LINDA M PARYSEK
• 金额: $ 36.74万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287986
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; charge coupled device camera; transmission electron microscopy

An active group of NIH-funded investigators is requesting a new transmission electron microscope, a JEOL JEM-1230, to replace our current microscope, a 15-year-old JEOL 100CXII. Electron microscopy is an integral part of the research programs of the eight Major Users on this application, who have a collective total of 139 years of electron microscopy experience. A new, state-of-the-art instrument would meet these investigators' needs for technology available on recently-designed transmission electron microscopes that cannot be provided by any microscope currently housed on campus or in neighboring institutions. The proposed new microscope is much easier to use than our current JEOL 100CXII and has many features that will allow users to capture higher-quality images much faster. In addition, the new microscope provides capabilities, not found on our current electron microscope that meet investigator's needs for quantitative analysis. Specifically, the JEM- 1230 permits three kinds of on-screen measurements. Six Major Users must make more elaborate measurements. We propose, therefore, to insert a CCD camera, for which the JEM-1230 is properly constructed, that would allow for capture of digital images that can be imported directly into image analysis programs. Digital image capture would circumvent the current tedious and time-consuming task of developing standard negatives and then digitizing them for subsequent analysis. Acquisition of the JEM-1230 and accessory CCD camera would provide a significant technological improvement in our electron microscope facility and allow investigators to more rapidly and efficiently complete the Specific Aims of their research programs.

一个由 NIH 资助的研究人员组成的活跃小组正在请求一种新型透射电子显微镜 JEOL JEM-1230，以取代我们现有的显微镜（已有 15 年历史的 JEOL 100CXII）。电子显微镜是该应用的八个主要用户研究计划的一个组成部分，他们总共拥有 139 年的电子显微镜经验。一种新的、最先进的仪器将满足这些研究人员对最近设计的透射电子显微镜技术的需求，而目前校园或邻近机构中的任何显微镜都无法提供这些技术。拟议的新型显微镜比我们当前的 JEOL 100CXII 更容易使用，并且具有许多功能，可以让用户更快地捕获更高质量的图像。此外，新型显微镜还提供了当前电子显微镜所没有的功能，可以满足研究人员的定量分析需求。具体来说，JEM-1230 允许三种屏幕测量。六大用户必须进行更精细的测量。因此，我们建议插入一个 CCD 相机（JEM-1230 已为其正确构建），以便捕获可直接导入图像分析程序的数字图像。数字图像捕获将避免当前开发标准底片然后将其数字化以供后续分析的繁琐且耗时的任务。购买 JEM-1230 和附件 CCD 相机将为我们的电子显微镜设施带来重大的技术改进，并使研究人员能够更快、更高效地完成其研究项目的具体目标。