TRANSMISSION ELECTRON MICROSCOPE

透射电子显微镜

• 批准号: 6287986
• 负责人: LINDA M PARYSEK
• 金额: $ 36.74万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287986
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; charge coupled device camera; transmission electron microscopy

An active group of NIH-funded investigators is requesting a new transmission electron microscope, a JEOL JEM-1230, to replace our current microscope, a 15-year-old JEOL 100CXII. Electron microscopy is an integral part of the research programs of the eight Major Users on this application, who have a collective total of 139 years of electron microscopy experience. A new, state-of-the-art instrument would meet these investigators' needs for technology available on recently-designed transmission electron microscopes that cannot be provided by any microscope currently housed on campus or in neighboring institutions. The proposed new microscope is much easier to use than our current JEOL 100CXII and has many features that will allow users to capture higher-quality images much faster. In addition, the new microscope provides capabilities, not found on our current electron microscope that meet investigator's needs for quantitative analysis. Specifically, the JEM- 1230 permits three kinds of on-screen measurements. Six Major Users must make more elaborate measurements. We propose, therefore, to insert a CCD camera, for which the JEM-1230 is properly constructed, that would allow for capture of digital images that can be imported directly into image analysis programs. Digital image capture would circumvent the current tedious and time-consuming task of developing standard negatives and then digitizing them for subsequent analysis. Acquisition of the JEM-1230 and accessory CCD camera would provide a significant technological improvement in our electron microscope facility and allow investigators to more rapidly and efficiently complete the Specific Aims of their research programs.

一组由NIH资助的研究人员要求使用新的传输电子显微镜JEOL JEM-1230，以取代我们当前的显微镜，这是15岁的JEOL 100CXII。电子显微镜是该应用程序中八个主要用户的研究计划的组成部分，他们拥有139年的电子显微镜体验。一种新的，最先进的工具将满足这些调查人员对最近设计的传输电子显微镜可用的技术需求，这些技术无法由当前在校园或邻近机构中的任何显微镜提供。提出的新显微镜比我们当前的JEOL 100CXII更容易使用，并且具有许多功能，可以使用户更快地捕获更高质量的图像。此外，新的显微镜提供了能力，在我们当前的电子显微镜上找不到能力，可以满足研究者对定量分析的需求。具体而言，JEM-1230允许三种屏幕测量。六个主要用户必须进行更精细的测量。因此，我们建议插入适当构建JEM-1230的CCD摄像机，该摄像头可以捕获可以直接导入到图像分析程序中的数字图像。数字图像捕获将规定当前繁琐且耗时的任务，即制定标准负面因素，然后将其数字化以进行后续分析。获得JEM-1230和配件CCD摄像头的获取将为我们的电子显微镜设施提供显着的技术改进，并允许研究人员更快，更有效地完成其研究计划的具体目标。