Purchase of a large format CCD camera for 3-D EM

购买用于 3-D EM 的大幅面 CCD 相机

• 批准号: 6877307
• 负责人: KENNETH ALLEN TAYLOR
• 金额: $ 24.03万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877307
• 关键词: Adenoviridae; HIV infections; bioimaging /biomedical imaging; biomedical equipment purchase; cell adhesion; charge coupled device camera; cryoelectron microscopy; electron microscopy; simian immunodeficiency virus; sperm motility; three dimensional imaging /topography

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a large format CCD camera to enhance 3-D electron microscopy at Florida State University. The camera will be mounted on a Philips/FEI CM300-FEG and replace an existing 2k x 2k CCD camera which will be transferred to a CM120. Both microscopes and both CCD cameras are housed in a shared university facility, called the Biological Science Imaging Resource (BSIR), which contains instrumentation for both light and electron microscopy used by many researchers on campus and in the north Florida area. The BSIR is professionally staffed by employees that are paid by the university, not by user fees, thereby providing substantial support for users who only pay fees to cover service contracts and expendable supplies. NIH funded projects that will be enhanced by this equipment include the following: electron tomography of Ascaris sperm motility complexes, SIV and HIV viruses, insect flight muscle, cell adhesion complexes and adeno-associated virus. In addition the camera will find use in various cryoEM 3-D imaging projects that require high throughput data collection. These include adeno-associated virus in complex with various receptors, heavy meromyosin in many different states, membrane proteins from Mycobacterium tuberculosis, and 2-D arrays of various kinds formed on lipid monolayers. The new instrumentation will allow for development of algorithms for increasing the area that can be reconstructed by electron tomography which may need corrections for focus gradient and for imaging distortions.

描述（由申请人提供）： 要求购买大型CCD摄像头的资金来增强佛罗里达州立大学的3-D电子显微镜。该相机将安装在飞利浦/FEI CM300 feg上，并更换现有的2K x 2K CCD相机，该相机将转移到CM120。显微镜和两个CCD摄像机都位于共享的大学设施中，称为生物科学成像资源（BSIR），其中包含许多校园和北佛罗里达州许多研究人员使用的光和电子显微镜的仪器。 BSIR专业由大学支付的员工（而不是通过用户费用），从而为仅支付费用以涵盖服务合同和可消耗品的用户提供了可观的支持。 NIH资助的项目将通过此设备增强，包括以下内容：Ascaris精子运动复合物，SIV和HIV病毒，昆虫飞行肌肉，细胞粘附复合物和腺相关病毒的电子断层扫描。此外，相机将在需要高吞吐量数据收集的各种冷冻3-D成像项目中找到使用。这些包括与各种受体相关的腺相关病毒，许多不同状态的沉重毛霉素，结核分枝杆菌的膜蛋白以及在脂质单层形成的各种种类的2-D阵列。新的仪器将允许开发算法，以增加可以通过电子断层扫描重建的区域，该区域可能需要校正焦点梯度和成像变形。