Identifying Genes Involved in Neuronal Differentiation

鉴定参与神经元分化的基因

• 批准号: 6641141
• 负责人: BIN CHEN
• 金额: $ 4.99万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6641141
• 关键词: developmental genetics; developmental neurobiology; gene induction /repression; gene targeting; genetically modified animals; laboratory mouse; microarray technology; neurogenesis; neurogenetics; subtraction hybridization; transcription factor; visual cortex

DESCRIPTION (provided by applicant): The mammalian cerebral cortex is a layered structure. I am interested in the molecules that regulate development of cortical layers. The first specific aim is to identify genes that are critical in the formation of proper axonal projection patterns for neurons within a specific cortical layer, layer 5. Layer 5 neurons initially extend axonal branches to many subcortical areas. Later the exuberant branches are selectively pruned so that a proper area-specific projection pattern is formed. The homeodomain transcription factor, Otx1, is required for the pruning of exuberant axonal branches. However nothing is known about the target genes of Otx l or about the mechanisms of axonal pruning. I propose to identify genes that act downstream of Otx1 to regulate axonal refinement using cDNA microarray technology. The second aim is to isolate and study genes which regulate the initial laminar fate determination or early stages of laminar differentiation. I have finished an in situ hybridization screening and identified twelve novel as well as known genes that demonstrate layer-specific expression patterns. Experiments are proposed to study their potential functions in laminar fate determination and/or early stages of layer differentiation.

描述（由申请人提供）：哺乳动物大脑皮层是分层的 结构。我对调节发育的分子感兴趣 皮质层。 第一个具体目标是识别在形成中至关重要的基因 特定皮质内神经元的正确轴突投射模式 第 5 层。第 5 层神经元最初将轴突分支延伸到许多 皮质下区域。随后有选择地修剪茂盛的枝条，以便 形成适当的特定区域投影图案。同源域 转录因子 Otx1 是修剪旺盛轴突所必需的 分支机构。然而，对于 Otx l 的靶基因或关于 轴突修剪机制。我建议找出作用于下游的基因 使用 cDNA 微阵列技术调节 Otx1 的轴突细化。 第二个目标是分离和研究调节初始层流的基因。 命运决定或层状分化的早期阶段。我已经完成了 原位杂交筛选并鉴定出 12 种新的和已知的 表现出层特异性表达模式的基因。实验是 提议研究它们在层状命运决定中的潜在功能 和/或层分化的早期阶段。