Connections and function of reproductive neural circuits

生殖神经回路的连接和功能

• 批准号: 7155754
• 负责人: TODD Erryl ANTHONY
• 金额: $ 5.04万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-18 至 2009-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7155754
• 关键词: DNA binding protein; amygdala; behavioral genetics; central neural pathway /tract; cholera toxin; developmental neurobiology; ethology; fluorescent dye /probe; fos protein; gene induction /repression; gene targeting; gold; hormone regulation /control mechanism; immunofluorescence technique; laboratory mouse; neuroanatomy; neurogenetics; neuronal transport; olfactory lobe; olfactory stimulus; postdoctoral investigator; radiotracer; sex behavior

DESCRIPTION (provided by applicant): The medial amygdala posterior dorsal (MEApd) and principal nucleus of the bed nucleus of the stria terminalis (BSTpr) are thought to be critical for integrating hormonal and olfactory signals and modulating reproductive behavior accordingly. Our long term goal is to elucidate the connectivity and function of genetically defined MEApd and BSTpr neuronal subpopulations in order to determine the specific circuits and mechanisms responsible for translating hormonal and chemosensory inputs into specific behavioral outputs. The specific hypothesis to be tested in this proposal is that MEApd and BSTpr contain multiple functionally distinct neuronal subpopulations, each of which make essential but unique contributions to reproductive behavior. This hypothesis will be tested via the following three specific aims: 1) Use classical neuroanatomical tracing to determine if multiple neuronal subpopulations with distinct connectivity exist in MEApd and BSTpr; 2) Determine if MEApd receives input from both the AOB and MOB using Cre-dependent transneuronal tracers; 3) Use genetic ablations to test the hypothesis that distinct MEApd and BSTpr neuronal subpopulations serve unique functional roles in modulating male reproductive behavior.

描述（由申请人提供）：内侧杏仁核后背侧（MEApd）和终纹床核主核（BSTpr）被认为对于整合激素和嗅觉信号并相应调节生殖行为至关重要。我们的长期目标是阐明基因定义的 MEApd 和 BSTpr 神经元亚群的连接性和功能，以确定负责将激素和化学感应输入转化为特定行为输出的特定电路和机制。该提案要测试的具体假设是 MEApd 和 BSTpr 包含多个功能不同的神经元亚群，每个亚群对生殖行为都做出重要但独特的贡献。该假设将通过以下三个具体目标进行检验：1）使用经典神经解剖学追踪来确定 MEApd 和 BSTpr 中是否存在具有不同连接性的多个神经元亚群； 2) 使用依赖于 Cre 的跨神经元示踪剂确定 MEApd 是否接收来自 AOB 和 MOB 的输入； 3) 使用基因消融来检验以下假设：不同的 MEApd 和 BSTpr 神经元亚群在调节男性生殖行为中发挥独特的功能作用。