CCD Digital Imaging System for Core EM Facility

核心 EM 设施的 CCD 数字成像系统

• 批准号: 6731596
• 负责人: ROGER W CRAIG
• 金额: $ 19.85万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731596
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; charge coupled device camera; cryoelectron microscopy; digital imaging; transmission electron microscopy

DESCRIPTION (provided by applicant): This application is a request for funds to provide CCD digital imaging capability for 11 major and 5 minor users of TEMs in the Core Electron Microscopy Facility of the University of Massachusetts Medical School (UMMS). Ultrastructural projects range from the tissue to the molecular level, and include low dose, tomographic, cryo-EM, serial section, immunolabeling, and morphological studies of a wide range of biomedically important systems. The major benefit of direct digital image acquisition is the instantaneous feedback that it provides, in the form of immediate images and instant knowledge of imaging parameters and specimen characteristics. These benefits make a critical difference to specialist applications, such as cryo-EM and tomography, where they can save hundreds of hours of work. In some cases studies become possible for the first time using CCD technology. The benefits of CCD imaging are also key to enhancing productivity in more routine applications. Digital acquisition not only adds convenience and saves time (by eliminating the film processing, printing, and scanning necessary for analysis and publication of images), but also results in major cost savings. In the past year, UMMS researchers spent approximately $40,000 in supplies and personnel costs for developing, printing and scanning the approximately 10,000 micrographs that were taken, all on film. Digital imaging would have eliminated almost all of these costs. Finally, digital imaging is important in teaching, facilitating instruction of novice microscopists and providing stimulating images for visiting medical, graduate and high school students. To provide for the needs of both specialist and routine TEM users alike, we are requesting funds to acquire digital imaging capability for two microscopes. These microscopes perform complementary tasks: one is reserved specifically for specialist applications, including low dose microscopy, cryo-EM and tomography, while the other is used for routine microscopy. This division of labor avoids any compromise to the functioning of the specialist microscope, where optimal optical, vacuum and mechanical performance are crucial. Equipping both microscopes with slow scan CCD cameras will extend the advantages of this technology to all researchers who require it. A third TEM will continue to operate with film for researchers who prefer this medium.

描述（由申请人提供）：此申请是为马萨诸塞大学医学院（UMMS）的核心电子显微镜设施中11个主要和5个次要TEMS提供CCD数字成像能力的资金。超微结构项目的范围从组织到分子水平，包括低剂量，层析成像，冷冻EM，串行部分，免疫标记以及多种生物医学重要系统的形态学研究。 直接数字图像采集的主要好处是它以直接图像的形式以及对成像参数和标本特征的即时知识的形式提供的瞬时反馈。这些好处对诸如Cryo-EM和层析成像等专业应用具有至关重要的影响，它们可以节省数百小时的工作。在某些情况下，使用CCD技术是首次进行研究。 CCD成像的好处也是提高常规应用中生产率的关键。数字收购不仅增加了便利并节省了时间（通过消除胶卷处理，打印和扫描图像的分析和发布所需的扫描），还可以节省大量成本。在过去的一年中，UMMS研究人员花费了大约40,000美元的供应和人员费用来开发，打印和扫描大约10,000个拍摄的显微照片。数字成像将消除几乎所有这些成本。最后，数字成像对于教学，促进新手显微镜的教学以及为访问医学，研究生和高中生提供刺激的图像很重要。 为了满足专家和常规TEM用户的需求，我们要求资金以获得两个显微镜的数字成像能力。这些显微镜执行互补任务：一个专门用于专业应用，包括低剂量显微镜，冷冻EM和层析成像，而另一个用于常规显微镜。这种劳动分裂避免了对专业显微镜功能的任何妥协，在该功能中，最佳的光学，真空和机械性能至关重要。为两个显微镜配备缓慢的扫描CCD摄像头将将这项技术的优势扩展到所有需要它的研究人员。对于喜欢这种媒介的研究人员，第三个TEM将继续与电影一起运作。