Transmission Electron Microscope for Core EM Facility

核心 EM 设施的透射电子显微镜

• 批准号: 7794260
• 负责人: ROGER W CRAIG
• 金额: $ 50万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-20 至 2010-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794260
• 关键词: Apoptotic; Architecture; Area; Arts; Biomedical Research; Boston; Cell Death; Cells; Cellular Structures; Centrosome; Cilia; Computer Interface; Development; Disease; Documentation; Electron Microscope; Ensure; Flagella; Funding; Future; G-Protein-Coupled Receptors; Gold; Hand; Housing; Image; Infection; Knowledge; Label; Location; MAP Kinase Signaling Pathways; Maintenance; Massachusetts; Microscope; Modernization; Molecular; Monitor; Nerve Degeneration; Play; Problem Solving; Productivity; Regulation; Research Personnel; Resolution; Role; Spliceosomes; Staging; Striated Muscles; Structure; Technology; Universities; base; charge coupled device camera; human disease; insight; instrument; medical schools; receptor structure function; synaptogenesis; tool; transmission process; user-friendly

DESCRIPTION (provided by applicant): We request funds for the purchase of an FEI Tecnai G2 Spirit BioTwin transmission electron microscope (TEM) with embedded CCD camera. This will serve the biomedical research needs of eleven NIH-funded investigators at the University of Massachusetts Medical School (UMMS) and Boston University School of Medicine. The microscope will be housed in the Core EM Facility of UMMS, and maintained and monitored by the Facility Manager, ensuring efficient use by investigators. It will replace a 23-year old, second-hand Philips CM12, which is based on outdated technology, lacks key capabilities required by users, and for which maintenance is likely to be discontinued or become difficult in the near future. The new instrument would immediately solve these problems. The projects that will use the Tecnai include fundamental studies of autophagic and apoptotic cell death, mechanisms of synapse formation, the regulation of contraction in smooth and striated muscle, the role of MAP kinase signaling pathways in neurodegeneration, the function of centrosomes in normal and diseased cells, the regulation of G-protein-coupled receptors, the structure and function of spliceosomes, and the structure and function of cilia and flagella in normal and diseased states. These projects require TEM as an essential tool for investigating cellular and molecular architecture at high resolution and as a means of detecting and localizing specific cell molecules by immuno-gold labeling. The images obtained will provide essential insights into mechanisms of cell development, function, infection and disease. In addition to the users in this application, there are numerous other investigators at UMMS who use TEM on a more occasional basis and who would also benefit from modernization of our TEM technology. The instrument proposed represents the state-of-the-art for a conventional TEM, and has many features that would greatly enhance TEM productivity at UMMS. These include a modern, user-friendly computer interface, motorized stage controls with 180o tilt capability, the ability to store and recall grid locations and alignment and imaging parameters (especially useful in several studies requiring serial sectioning), and full integration of an embedded CCD camera, making it possible to record images rapidly and with full documentation. All of the projects that will use the microscope aim to provide fundamental insights into cellular structure and function. Most have a direct bearing on the understanding of human disease. Access to this state-of-the-art instrument will play a key role in advancing our knowledge in these biomedically important areas.

描述（由申请人提供）：我们要求购买带有嵌入式CCD摄像机的Fei Tecnai G2 Spirit Electron Electron显微镜（TEM）的资金。这将满足马萨诸塞大学医学院（UMMS）和波士顿大学医学院的11名NIH资助的研究人员的生物医学研究需求。显微镜将安置在UMMS的核心EM设施中，并由设施经理维护和监视，以确保研究人员有效使用。它将取代基于过时的技术，它将取代23岁的二手飞利浦CM12，它缺乏用户所需的关键功能，并且在不久的将来可能会停产或变得困难。新工具将立即解决这些问题。 将使用tecnai的项目包括对自噬和凋亡细胞死亡的基本研究，突触形成的机制，平滑和横纹肌肉中收缩的调节，神经变性中的MAP激酶信号通路在神经变性中的作用，正常和疾病细胞中的中心体的函数，正常细胞中的中心体的功能，结构和结构的受体，跨越结构和功能，以及跨越的结构和功能，该功能，功能，功能，功能，功能，功能，起作用的功能，起作用的功能和功能，起作用的功能和功能，起作用的功能和功能，起作用的功能，起作用的功能，以及功能的功能，是centrosomes的调节。正常和患病状态的纤毛和鞭毛。这些项目需要TEM作为在高分辨率下研究细胞和分子结构的必要工具，并通过免疫金标记来检测和定位特定细胞分子的手段。获得的图像将提供有关细胞发育，功能，感染和疾病机制的基本见解。 除了本应用程序中的用户外，UMMS上还有许多其他调查员会更偶尔使用TEM，并且还将从我们的TEM技术的现代化中受益。提出的仪器代表了常规TEM的最新设备，并且具有许多功能，可以极大地提高UMMS的TEM生产率。其中包括现代用户友好的计算机接口，具有180o倾斜功能的电动舞台控件，能够存储和回忆网格位置以及对齐地点和成像参数（在需要串行分段的几项研究中尤其有用），以及嵌入式CCD摄像头的完全集成，使得可以快速记录图像并完整文档记录图像。 所有将使用显微镜的项目旨在提供对细胞结构和功能的基本见解。大多数人对人类疾病的理解有直接关系。访问这种最先进的工具将在促进我们在这些重要重要领域的知识方面发挥关键作用。

项目成果

The secreted neurotrophin Spätzle 3 promotes glial morphogenesis and supports neuronal survival and function.

• DOI: 10.1101/gad.305888.117
• 发表时间: 2017-10-15
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Coutinho-Budd JC;Sheehan AE;Freeman MR
• 通讯作者: Freeman MR

Neuron-glia interactions through the Heartless FGF receptor signaling pathway mediate morphogenesis of Drosophila astrocytes.

• DOI: 10.1016/j.neuron.2014.06.026
• 发表时间: 2014-07-16
• 期刊: NEURON
• 影响因子: 16.2
• 作者: Stork, Tobias;Sheehan, Amy;Tasdemir-Yilmaz, Ozge E.;Freeman, Marc R.
• 通讯作者: Freeman, Marc R.

Astrocytes engage unique molecular programs to engulf pruned neuronal debris from distinct subsets of neurons.

• DOI: 10.1101/gad.229518.113
• 发表时间: 2014-01-01
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Tasdemir-Yilmaz OE;Freeman MR
• 通讯作者: Freeman MR

Robust Distal Tip Cell Pathfinding in the Face of Temperature Stress Is Ensured by Two Conserved microRNAS in Caenorhabditis elegans.

• DOI: 10.1534/genetics.115.179184
• 发表时间: 2015-08
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Burke SL;Hammell M;Ambros V
• 通讯作者: Ambros V

Temporal evolution of susceptibility artifacts from coiled aneurysms on MR angiography: an in vivo canine study.

MR 血管造影上盘绕动脉瘤敏感性伪影的时间演变：一项犬类体内研究。

• DOI: 10.3174/ajnr.a2831
• 发表时间: 2012
• 期刊: AJNR. American journal of neuroradiology
• 作者: Spilberg,G;Carniato,SL;King,RM;vanderBom,IMJ;Mehra,M;Walvick,RP;Wakhloo,AK;Gounis,MJ
• 通讯作者: Gounis,MJ