JSM-6335F FE SCANNING ELECTRON MICROSCOPE

JSM-6335F FE扫描电子显微镜

• 批准号: 6288123
• 负责人: SIMON C WATKINS
• 金额: $ 31.76万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288123
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; scanning electron microscopy

This request is for partial funds to purchase a JEOL 6335E Scanning Electron Microscope (SEM). This instrument will serve as only SEM within the Center for Biologic Imaging (CBI) at the University of Pittsburgh Medical School. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the medical area. SEM is an essential service provided by the center. The current microscope within the facility is a 15 year old JEOL T300. Over the last several years it has become apparent to the steering committee of the CBI that this microscope is inadequate in terms of both reliability, resolution and flexibility. The specific technical concerns of users are that; the instrument is unreliable, it offers only the most limited resolution, no possibility exists to collect backscattered electron images, and no possibility for direct digital image collection and archive exist. Since the CBI commenced operations 8 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 50 PHS funded groups within the medical area. Over the last few years field emission guns SEMs have become increasingly easy to align and maintain which has allowed their use within biomedical research to expand enormously, to include truly high resolution imaging of surfaces as well as the possibility of imaging specific surface labeling sites at high resolution. There is simply no possibility of performing this type of study anywhere on the campus of the University of Pittsburgh medical school, in fact the microscope within the CBI serves the needs of not only the medical school users but also federally funded users in Bioengineering, and Biological Sciences. The limitations of the current microscope leads to considerable frustration amongst users. The acquisition of the requested instrument, which offers all the principally requested features in a reasonably priced instrument is a critical component in the expansion of imaging technologies available to PHS supported users at the University of Pittsburgh Medical Center.

此请求用于购买 JEOL 6335E 扫描电子显微镜 (SEM) 的部分资金。该仪器将作为匹兹堡大学医学院生物成像中心 (CBI) 内唯一的 SEM。该核心设施的任务是为医学领域内的所有研究小组提供全方位的光和电子光学、图像分析和形​​态测量方法。 SEM是该中心提供的一项重要服务。该设施内当前使用的显微镜​​是一台已有 15 年历史的 JEOL T300。在过去的几年里，CBI 指导委员会已经清楚地认识到这种显微镜在可靠性、分辨率和灵活性方面都存在不足。用户的具体技术问题是：该仪器不可靠，它仅提供最有限的分辨率，不可能收集反向散射电子图像，也不可能进行直接数字图像收集和存档。自 8 年前开始运作以来，CBI 已成为医学研究界不可分割的一部分，目前与医学领域内 50 多个 PHS 资助的团体一起参与研究项目。在过去的几年中，场发射枪 SEM 变得越来越容易对准和维护，这使得它们在生物医学研究中的使用得到了极大的扩展，包括真正高分辨率的表面成像以及在高光下对特定表面标记位点进行成像的可能性。解决。在匹兹堡大学医学院校园的任何地方根本不可能进行此类研究，事实上，CBI 内的显微镜不仅满足医学院用户的需求，还满足联邦资助的生物工程和生物领域用户的需求。科学。当前显微镜的局限性导致用户相当沮丧。购买所需的仪器，以价格合理的仪器提供所有主要要求的功能，是扩展匹兹堡大学医学中心 PHS 支持用户可用的成像技术的关键组成部分。