PHILIPS XL30 SCANNING ELECTRON MICROSCOPE

飞利浦 XL30 扫描电子显微镜

• 批准号: 6052084
• 负责人: ROBERT S MCCUSKEY
• 金额: $ 13.86万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052084
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; scanning electron microscopy

There are severe limitations for conventional scanning electron microscopy of biological material. These include limitations on resolution, lack of back-scattered detection, and the inability to image uncoated, non-conductive samples at low accelerating voltages. All of these capabilities are critically needed by a number of NIH funded investigators and lack of access to them at the University of Arizona is seriously limiting our productivity and progress. As a result, we are requesting funds to purchase a Philips XL30 SEM equipped with a conventional secondary electron detector for optimal detection under high vacuum conditions, and a backscattered electron detector. The Philips XL30 SEM was selected as the instrument on the market having the features which make it the most suitable instrument for the needs of our major user group. These needs include the ability to: (a) obtain high resolution secondary electron images at low voltage on the surfaces of fixed, critical-point dried, but uncoated sample, (c) obtain backscattered electron images of uncoated samples to reveal internal structure as well as the distribution heavy metal (e.g., colloidal gold) labeling of surface structures; (d) record these images in photographic video, or digital format for subsequent presentation, archiving, and/or image analysis. The research plans demonstrate our immediate and critical need for an SEM not only to replace our now defunct, 22 year-old conventional SEM at the College of Medicine Imaging Faculty, but also to provide high-resolution conventional SEM capabilities to eight NIH-funded major users, and at least six NIH-funded minor users in a variety of departments within the University of Arizona and one investigator from the University of Southern California who collaborates with the Principal Investigator for the electron microscopy required for her project.

生物材料的传统扫描电子显微镜存在严重的局限性。这些问题包括分辨率的限制、缺乏背向散射检测以及无法在低加速电压下对未涂覆的非导电样品进行成像。所有这些能力都是 NIH 资助的许多研究人员迫切需要的，而亚利桑那大学缺乏这些能力严重限制了我们的生产力和进步。因此，我们请求资金购买配备传统二次电子探测器（可在高真空条件下实现最佳检测）和背散射电子探测器的飞利浦 XL30 SEM。飞利浦 XL30 SEM 被选为市场上的仪器，其功能使其成为最适合我们主要用户群需求的仪器。这些需求包括以下能力：(a) 在低电压下在固定、临界点干燥但未涂覆的样品表面获得高分辨率二次电子图像，(c) 获得未涂覆样品的背散射电子图像以揭示内部结构作为表面结构的分布重金属（例如胶体金）标记； (d) 以摄影视频或数字格式记录这些图像，以供后续演示、存档和/或图像分析。研究计划表明，我们对 SEM 的迫切需求不仅是为了取代医学影像学院现已不存在的、已有 22 年历史的传统 SEM，而且还为 NIH 资助的八个专业提供高分辨率的传统 SEM 功能用户，以及亚利桑那大学各个部门的至少六名 NIH 资助的未成年用户，以及南加州大学的一名研究人员，她与首席研究员合作开发其项目所需的电子显微镜。