MASS SPECTROMETERS

质谱仪

• 批准号: 2516741
• 负责人: RUTH Hogue ANGELETTI
• 金额: $ 20万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 1999-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2516741
• 关键词: biomedical equipment; biomedical equipment purchase; electrospray ionization mass spectrometry; mass spectrometry

This proposal requests funds for the acquisition of two mass spectrometers, the Perseptive DE STR and the Finnigan LC-Q instruments, plus the nanoelectrospray ionization source for both the Finnigan LC-Q and the existing Sciex API-III mass spectrometers, for the analysis of biological molecules for research programs at the Albert Einstein College of Medicine. The instruments will be used within the context of an established, productive resource laboratory with documented experience in interfacing mass spectrometry to basic biomedical research projects and in interacting with diverse groups of investigators. The rationale for assembling the suite of mass spectrometry instruments, including those requested in this proposal, is the need for higher mass accuracies and sensitivities for present and future projects, the number and complexity of established and developing projects, and the mass spectrometry experience of the personnel. Major projects address important issues in signal transduction, protein processing and sorting, immunology, mechanistic enzymology, protein structure-function analysis, and analysis of nucleic acids. We wish to address the advanced analytical needs of existing research programs and the challenging questions in experimental biology now being developed.

该建议要求资金获得两个群众 光谱仪，具有意义的de str和Finnigan LC-Q仪器， 再加上Finnigan LC-Q的纳米电喷雾电离源 以及现有的SCIEX API-III质谱仪，用于分析 爱因斯坦研究计划的生物分子 医学院。这些仪器将在 一个已建立的生产资源实验室，有记录的 将质谱与基本生物医学研究接口的经验 项目并与不同的调查人员互动。 这 组装质谱仪器套件的理由， 包括本提案中要求的那些是需要更高的质量 对当前和未来项目的精确和敏感性，数字 以及已建立和发展项目的复杂性以及群众 人员的光谱经验。 主要项目地址 信号转导，蛋白质处理和分类的重要问题， 免疫学，机械酶学，蛋白质结构功能分析， 和核酸的分析。 我们希望解决高级 现有研究计划的分析需求和具有挑战性 现在已经开发了实验生物学的问题。