High resolution triple quadrupole mass spectrometer

高分辨率三重四极杆质谱仪

• 批准号: 6876978
• 负责人: Ian Alexander Blair
• 金额: $ 40.43万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-03 至 2006-09-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876978
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; mass spectrometry

DESCRIPTION (provided by applicant): High Resolution Triple Quadrupole Mass Spectrometer: Funds are requested from the Division of Research Resources for the purchase of Thermo Finnigan TSQ Quantum Ultra AM high-resolution triple quadrupole MS/MS system. Until the development this instrument, no high-resolution triple quadrupole mass spectrometers have been available. The use of conventional triple quadrupole instruments has become an important tool in laboratories that are engaged in biomarker research using quantitative mass spectrometry. However, it is becoming recognized that the ability to analyze molecules in the electron capture mode atmospheric pressure chemical ionization (APCI) mode that we discovered several years ago offers significant advantages when compared with conventional ionization methods for the analysis of endogenous compounds. In fact over thirty applications of this methodology have already cited our original discovery. The ability to conduct analyses with high resolution and high sensitivity provides an additional advantage over conventional triple quadrupole methodology. The new Ion Max source in this instrument has interchangeable electrospray ionization (ESI) and APCI probes, a titanium skimmer with hard-edged skimming, and larger aperture for increase robustness and sensitivity. It has a redesigned ion transfer tube lens with wider aperture for enhanced ion focusing and ion transmission. The "Ion Max" source also allows full probe adjustment in the x, y, and z directions with two view ports and a quick release probe lever. The probe position can be optimized for maximum robustness and sensitivity with any given combination of flow rate, solvent, and ionization mode. Its new, ultra high temperature, self-cleaning APCI probe, with ceramic heater, virtually eliminates carryover. The Finnigan TSQ Quantum Ultra's innovative technology provides the widest linear dynamic range and lowest sensitivity 1imits o f any triple quadrupole mass spectrometer on the market (5-6 orders of magnitude). The instrument has the powerful capability of routine accurate mass measurements on the chromatographic timescale. Its redesigned analyzer control circuitry allows full advantage to be taken of the high-resolution HyperQuad mass analyzers. This makes it possible to conduct high-resolution analyses with minimal loss of sensitivity. The use of electron capture APCI coupled with high resolution and high sensitivity offers unique capabilities for lipid, DNA, and protein biomarkers of oxidative stress. The Thermo Finnigan TSQ Quantum Ultra AM LC/MS/MS System represents the current state-of-the-art in commercial instruments with electron capture APCI capabilities. A major user group consisting of five NIH-funded investigators would use the instrument to perform biomarker research. A minor user group consisting of five NIH-funded investigators would also conduct research using the instrument. An occasional user group of ten NIH-funded investigators would use the instrument for most of the remaining instrument time. It is estimated that over five years, the instrument would benefit the research programs of an additional 20-30 investigators. Therefore, the new instrument would impact significantly on research programs of some 40-50 NIH-funded investigators at the University of Pennsylvania.

描述（由申请人提供）： 高分辨率三重四极杆质谱仪：向研究资源部申请资金用于购买 Thermo Finnigan TSQ Quantum Ultra AM 高分辨率三重四极杆 MS/MS 系统。在开发该仪器之前，还没有高分辨率三重四极杆质谱仪。传统三重四极杆仪器的使用已成为利用定量质谱进行生物标志物研究的实验室的重要工具。然而，人们逐渐认识到，与分析内源性化合物的传统电离方法相比，我们几年前发现的电子捕获模式大气压化学电离 (APCI) 模式分析分子的能力具有显着的优势。事实上，这种方法的三十多个应用已经引用了我们最初的发现。与传统三重四极杆方法相比，进行高分辨率和高灵敏度分析的能力提供了额外的优势。该仪器中的新型 Ion Max 源具有可互换的电喷雾电离 (ESI) 和 APCI 探针、具有硬边撇渣的钛撇渣器以及用于提高稳健性和灵敏度的更大孔径。它具有重新设计的离子传输管透镜，孔径更宽，可增强离子聚焦和离子传输。 “Ion Max”源还允许通过两个观察端口和一个快速释放探针杆在 x、y 和 z 方向上进行完整的探针调整。可以针对任意给定的流速、溶剂和电离模式组合来优化探头位置，以实现最大的鲁棒性和灵敏度。其新型超高温自清洁 APCI 探头配有陶瓷加热器，几乎消除了残留。 Finnigan TSQ Quantum Ultra 的创新技术提供了市场上任何三重四极杆质谱仪中最宽的线性动态范围和最低的灵敏度 1 限制（5-6 个数量级）。该仪器具有在色谱时间尺度上进行常规精确质量测量的强大能力。其重新设计的分析仪控制电路可以充分利用高分辨率 HyperQuad 质量分析仪。这使得在灵敏度损失最小的情况下进行高分辨率分析成为可能。电子捕获 APCI 的使用与高分辨率和高灵敏度相结合，为氧化应激的脂质、DNA 和蛋白质生物标志物提供了独特的功能。 Thermo Finnigan TSQ Quantum Ultra AM LC/MS/MS 系统代表了具有电子捕获 APCI 功能的商业仪器的当前最先进水平。由五名 NIH 资助的研究人员组成的主要用户组将使用该仪器进行生物标志物研究。由五名美国国立卫生研究院资助的研究人员组成的小用户小组也将使用该仪器进行研究。由 10 名 NIH 资助的研究人员组成的临时用户组将在剩余的大部分仪器时间内使用该仪器。据估计，五年内，该仪器将使另外 20-30 名研究人员的研究项目受益。因此，新仪器将对宾夕法尼亚大学约 40-50 名 NIH 资助的研究人员的研究项目产生重大影响。