PROTEOMICS SHARED RESOURCE

蛋白质组学共享资源

• 批准号: 7506860
• 负责人: RUTH Hogue ANGELETTI
• 金额: $ 11.04万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-25 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7506860
• 关键词: Address; Albert Einstein Cancer Center; Analytical Chemistry; Area; Arts; Binding Sites; Bioinformatics; Biological; Biological Markers; Cancer Center; Cells; Commercial Sectors; Complex; Condition; Data Analyses; Deuterium; Development; Digestion; Enzyme Inhibitor Drugs; Enzyme Inhibitors; Equipment; Experimental Designs; Fluorescence; Gel; Goals; Head and neck structure; Human Resources; Hydrogen; Image Analysis; Laboratories; Malignant Squamous Cell Neoplasm; Mass Spectrum Analysis; Massachusetts; Membrane Proteins; Methodology; Microtubules; Online Systems; Peptide Synthesis; Peptides; Play; Post-Translational Protein Processing; Preparation; Protein Analysis; Protein Dynamics; Protein Isoforms; Proteins; Proteome; Proteomics; Proteomics Shared Resource; Qualifying; Recruitment Activity; Research; Research Personnel; Resource Sharing; Role; Sampling; Services; Solutions; Spottings; Technology; Temperature; Training; Tubulin; Two-Dimensional Gel Electrophoresis; Universities; Work; anticancer research; base; cancer risk; data mining; inhibitor/antagonist; insight; instrument; malignant breast neoplasm; mass spectrometer; member; protein expression; response; small molecule; therapeutic target

The Proteomics Shared Resource provides state-of-the-art protein technologies for cancer research with a focus on application of mass spectrometry and analytical chemistry. Services include: protein identification from samples in solution or in 1D or 2D gels; 2D gel electrophoresis, 2D-DIGE, image analysis, spot picking, and spot digestion; mass spectrometry analysis of protein complexes; membrane proteins; organellar proteomes and subproteomes; quantitative analysis of protein expression in different experimental conditions; posttranslational modifications; top-down analysis of proteins; development of biomarker and discovery projects; small molecule analysis by LC-MS, and hydrogen-deuterium exchange studies for protein dynamics. There is an adjacent computing area for offline mass spectrometry data analysis plus web-based searches and data mining. Major developments since the last review include a major renovation of the entire Proteomics facility and the installation of cooling equipment for temperature stabilization of instruments. Four new mass spectrometers (TOFTOF, LTQ linear trap, FT-ICR, QSTAR XL) were acquired along with a Typhoon fluorescence scanner. Several new highly qualified staff members have also been recruited. These include: Dr. Hui Xiao, an expert in FT-ICR mass spectrometry and hydrogen-deuterium exchange recruited from the University of Massachusetts, Amherst; Dr. Jihyeon Lim, also of Univ. of Mass., Amherst, an expert mass spectrometrist; and Dr. Fa-Yun Che, an expert in quantitative proteomics. Peptide synthesis was discontinued at the end of 2005 based upon the availability of high-quality peptides from companies in the commercial sector, allowing more effective utilization of personnel to support other critical services of high priority to users. The facility has played an important role in a variety of mass spectrometry applications that support high-priority AECC initiatives. These include development of transition-state enzyme inhibitors, the study of tubulin isoforms and microtubule-inhibitor binding sites, proteomic analyses as a predictor of breast cancer risk and for the prediction of response to treatment in squamous cell cancer of the head and neck. The facility has been active in the development of new methodologies to meet AECC investigator needs and in training AECC investigators so that they can use open-access laboratory equipment.

蛋白质组学共享资源为癌症研究提供最先进的蛋白质技术， 重点研究质谱和分析化学的应用。服务包括：蛋白质鉴定 来自溶液或一维或二维凝胶中的样品； 2D 凝胶电泳、2D-DIGE、图像分析、点选取、 和现场消化；蛋白质复合物的质谱分析；膜蛋白；细胞器 蛋白质组和亚蛋白质组；不同实验中蛋白质表达的定量分析 状况;翻译后修饰；自上而下的蛋白质分析；生物标志物的开发和 发现项目；通过 LC-MS 进行小分子分析，以及蛋白质的氢-氘交换研究 动力学。有一个相邻的计算区域，用于离线质谱数据分析和基于网络的分析 搜索和数据挖掘。自上次审查以来的主要进展包括对整个建筑进行重大翻新 蛋白质组学设施和安装冷却设备以稳定仪器的温度。四 购买了新的质谱仪（TOFTOF、LTQ 线性阱、FT-ICR、QSTAR XL）以及 台风荧光扫描仪。还招募了几名新的高素质工作人员。 其中包括： 肖辉博士，FT-ICR质谱和氢氘交换专家 从马萨诸塞大学阿默斯特分校招募； Jihyeon Lim 博士，也是大学的。马萨诸塞州阿默斯特， 质谱专家；定量蛋白质组学专家车发云博士。多肽合成 由于以下国家的公司提供高质量肽，该产品于 2005 年底停产： 商业部门，允许更有效地利用人员来支持其他关键服务 用户优先级高。该设施在各种质谱应用中发挥了重要作用 支持高度优先的 AECC 计划。其中包括过渡态酶抑制剂的开发， 微管蛋白亚型和微管抑制剂结合位点的研究，蛋白质组学分析作为预测因子 乳腺癌风险以及头部鳞状细胞癌治疗反应的预测 脖子。该机构一直积极开发新方法以满足 AECC 调查员的要求 并培训 AECC 调查员，使他们能够使用开放式实验室设备。