MUSERMET: MultiUSER equipment for small molecule identification in untargeted METabolomics

MUSERMET：用于非靶向代谢组学中小分子鉴定的 MultiUSER 设备

• 批准号: BB/S019030/1
• 负责人: Douglas Kell
• 金额: $ 61.16万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS019030%2F1
• 关键词: MUSERMET; MultiUSER; equipment; small; molecule

Many of the projects in the newly formed Liverpool Centre for Metabolomics Research involve a requirement for the sophisticated, detailed, and RAPID analysis of biological samples to identify and measure the many hundreds or thousands of small molecules (metabolites) that they contain. This is done by separating them in a flowing stream (liquid chromatography) followed by their estimation on the basis of their masses and the masses of any fragments that may be produced therefrom (as these form a unique 'signature'). Classically, a major bottleneck has been the IDENTIFICATION of the metabolites represented by what may otherwise be highly reproducible times of the appearance of these mass spectral peaks or signatures. An instrument called the Orbitrap ID-X tribrid, together with an integrated suite of software (to which we shall add), offers a real chance of solving this 'spectrum-to-structure' problem. It may be applied to the solution of multiple problems within the BBSRC remit, and we illustrate several. The aim of this proposal is to secure such an instrument for an extensive User Community in Liverpool and elsewhere. The instrument, while based on established Orbitrap technology, is both novel and unique, and no such instrument is currently available within the UK. This would be a 'first', and we have accordingly secured a substantial contribution from the applicant Universities.

新成立的利物浦代谢组学研究中心中的许多项目涉及对生物样品进行的复杂，详细和快速分析，以识别和测量其所含的数百或数千个小分子（代谢物）。这是通过将它们分离在流动的流（液相色谱）中，然后根据其质量和可能从其中产生的任何片段的质量进行估算（因为它们形成独特的“签名”）来完成。从经典上讲，主要的瓶颈是对这些质谱峰或特征的外观可能高度可重现的时间来代表的代谢物的鉴定。一种称为Orbitrap ID-X Tribrid的仪器，再加上集成的软件套件（我们将添加），提供了解决此“频谱到结构”问题的真正机会。它可以应用于BBSRC寄存中多个问题的解决方案，我们说明了几个问题。该提案的目的是为利物浦和其他地方的广泛用户社区保护这种工具。该仪器虽然基于既定的Orbitrap技术，既是新颖又独特的，并且目前在英国境内尚无此类工具。这将是一个“第一”，因此我们从申请人大学中获得了重大贡献。