MultiUSer equipment for high-throughput, high-content analysis in Industrial and Cellular biotechnology (MUSIC)

用于工业和细胞生物技术 (MUSIC) 中高通量、高内涵分析的多用户设备

• 批准号: BB/R000093/1
• 负责人: Douglas Kell
• 金额: $ 35.4万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR000093%2F1
• 关键词: MultiUSer; equipment; throughput; content; analysis

Many of the projects in the Manchester Institute of Biotechnology involve a requirement for the sophisticated, detailed, and above all RAPID analysis of biological cell samples. An instrument called the Intellicyt iQue Screener PLUS permits this, and the aim of this proposal is to secure such an instrument for an extensive User Community in Manchester and elsewhere. The instrument is unique (multiple patents) and no such instrument is available within 200 miles of Manchester.

曼彻斯特生物技术学院的许多项目涉及对生物细胞样品的复杂，详细，尤其是所有快速分析的要求。一种名为Intellicyt Ique Screener Plus的乐器允许此功能，该提案的目的是为曼彻斯特和其他地方的广泛用户社区保护这种工具。该乐器是独特的（多项专利），并且在曼彻斯特200英里内没有任何此类乐器。

项目成果

Very rapid flow cytometric assessment of antimicrobial susceptibility during the apparent lag phase of microbial (re)growth

• DOI: 10.1101/480392
• 发表时间: 2018-11
• 期刊: bioRxiv
• 作者: Srijan Jindal;H. Thampy;P. Day;D. Kell

A brain-permeable inhibitor of the neurodegenerative disease target kynurenine 3-monooxygenase prevents accumulation of neurotoxic metabolites.

神经退行性疾病的脑渗透性抑制剂以犬尿氨酸 3-单加氧酶为靶点，可防止神经毒性代谢物的积累。

• DOI: 10.1038/s42003-019-0520-5
• 发表时间: 2019
• 期刊: Communications biology
• 影响因子: 5.9
• 作者: Zhang S

A palette of fluorophores that are differentially accumulated by wild-type and mutant strains of Escherichia coli: surrogate ligands for profiling bacterial membrane transporters.

• DOI: 10.1099/mic.0.001016
• 发表时间: 2021-03
• 期刊: Microbiology (Reading, England)
• 作者: Salcedo-Sora JE;Jindal S;O'Hagan S;Kell DB
• 通讯作者: Kell DB

SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.

SpeedyGenesXL：一个自动化的高通量平台，用于为定向进化准备定制的超大型变体文库。

• DOI: 10.1007/978-1-0716-2152-3_5
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Sadler JC

Why most transporter mutations that cause antibiotic resistance are to efflux pumps rather than to import transporters

• DOI: 10.1101/2020.01.16.909507
• 发表时间: 2020-01-17
• 期刊: bioRxiv
• 作者: Mendes, P.;Girardi, E.;Kell, D. B.
• 通讯作者: Kell, D. B.